BMRB Entry 7179

Title:
Dispersin Solution Structure
Deposition date:
2006-06-21
Original release date:
2008-07-16
Authors:
Velarde, J.; Varney, K.; Inman, K.; Weber, D.; Nataro, J.
Citation:

Citation: Velarde, J.; Varney, K.; Farfan, M.; Dudley, E.; Inman, K.; Fletcher, J.; Weber, D.; Nataro, J.. "Solution structure of the novel dispersin protein of enteroaggregative Escherichia coli."  Mol. Microbiol. 66, 1123-1135 (2007).
PubMed: 17986189

Assembly members:

Assembly members:
Dispersin, Aap, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts380
15N chemical shifts96
1H chemical shifts539

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dispersin, Aap1

Entities:

Entity 1, Dispersin, Aap 104 residues - Formula weight is not available

1   GLYGLYSERGLYTRPASNALAASPASNVAL
2   ASPPROSERGLNCYSILELYSGLNSERGLY
3   VALGLNTYRTHRTYRASNSERGLYVALSER
4   VALCYSMETGLNGLYLEUASNGLUGLYLYS
5   VALARGGLYVALSERVALSERGLYVALPHE
6   TYRTYRASNASPGLYTHRTHRSERASNPHE
7   LYSGLYVALVALTHRPROSERTHRPROVAL
8   ASNTHRASNGLNASPILEASNLYSTHRASN
9   LYSVALGLYVALGLNLYSTYRARGALALEU
10   THRGLUTRPVALGLYSERARGSERHISHIS
11   HISHISHISHIS

Samples:

sample_1: Dispersin, Aap, [U-15N; U-13C], 1 mM; Phosphate 45 mM; NaCl 45 mM; EDTA 0.2 mM; NaN3 0.2 mM; DTT 0.1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
3D 13C-separated NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1
4D 13C-separated NOESYsample_1not availablesample_cond_1
4D 13C/15N-separated NOESYsample_1not availablesample_cond_1
3D 15N-edited HOHAHA-HSQCsample_1not availablesample_cond_1
3D 15N edited HMQC-NOESY-HSQCsample_1not availablesample_cond_1
3D 15N HNHAsample_1not availablesample_cond_1
3D HNCOsample_1not availablesample_cond_1
3D C(CO)NHsample_1not availablesample_cond_1
3D CBCA(CO)NHsample_1not availablesample_cond_1
3D HNCACBsample_1not availablesample_cond_1
3D HCA(CO)Nsample_1not availablesample_cond_1
IPAP-HSQCsample_1not availablesample_cond_1
3D HN(CO)CAsample_1not availablesample_cond_1

Software:

X-PLOR NIH v2.9.1 - refinement

NMRPipe v1.8 - processing

xwinnmr v2.6 - collection

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks