BMRB Entry 7174

Title:
Chemical Shift Assignments for Hdm2 RING finger domain
Deposition date:
2006-06-19
Original release date:
2006-10-30
Authors:
Kostic, Milka; Matt, Theresia; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter
Citation:

Citation: Kostic, Milka; Matt, Theresia; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter. "Solution Structure of Hdm2 C2H2C4 RING, a Domain Critical for Ubiquitination of p53"  J. Mol. Biol. 363, 433-450 (2006).
PubMed: 16965791

Assembly members:

Assembly members:
Hdm2 RING finger, polymer, 63 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hdm2 RING finger: SLPLNAIEPCVICQGRPKNG CIVHGKTGHLMACFTCAKKL KKRNKPCPVCRQPIQMIVLT YFP

Data sets:
Data typeCount
13C chemical shifts97
15N chemical shifts65
1H chemical shifts403

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hdm2 RING finger domain1
2ZINC (II) ION, 12
3ZINC (II) ION, 22
4Hdm2 RING finger domain1
5ZINC (II) ION, 12
6ZINC (II) ION, 22

Entities:

Entity 1, Hdm2 RING finger domain 63 residues - Formula weight is not available

1   SERLEUPROLEUASNALAILEGLUPROCYS
2   VALILECYSGLNGLYARGPROLYSASNGLY
3   CYSILEVALHISGLYLYSTHRGLYHISLEU
4   METALACYSPHETHRCYSALALYSLYSLEU
5   LYSLYSARGASNLYSPROCYSPROVALCYS
6   ARGGLNPROILEGLNMETILEVALLEUTHR
7   TYRPHEPRO

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Hdm2 RING finger, [U-13C; U-15N], 0.08 – 0.15 mM; NaCl 150 ± 5 mM; additive arginine 250 ± 5 mM; additive TCEP 0.5 mM; additive ZnSO4 0.005 mM

conditions_1: pH: 7.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
1H13C_HSQCsample_1not availableconditions_1
1H15N_3D_NOESY_HSQCsample_1not availableconditions_1
1H15N_3D_TOCSY_HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
1H15N_HMQCsample_1not availableconditions_1
2D_1H_NOESYsample_1not availableconditions_1
2D_1H_TOCSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 500 MHz
  • Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks