BMRB Entry 7119

Title:
NMR Assignment of the Human Cancer-Related Protein 32324
Deposition date:
2006-05-18
Original release date:
2006-10-02
Authors:
Placzek, William; Almeida, Marcius; Wuthrich, Kurt
Citation:

Citation: Placzek, William; Almeida, Marcius; Wuthrich, Kurt. "NMR Assignment of the Human Cancer-Related nucleoside triphosphatase"  J. Biomol. NMR 36, 59-59 (2006).
PubMed: 16933021

Assembly members:

Assembly members:
32324, polymer, 189 residues, 20581 Da.
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts789
15N chemical shifts171
1H chemical shifts1296

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
132324 monomer1
2Magnesium ion2

Entities:

Entity 1, 32324 monomer 189 residues - 20581 Da.

1   ALAARGHISVALPHELEUTHRGLYPROPRO
2   GLYVALGLYLYSTHRTHRLEUILEHISLYS
3   ALASERGLUVALLEULYSSERSERGLYVAL
4   PROVALASPGLYPHETYRTHRGLUGLUVAL
5   ARGGLNGLYGLYARGARGILEGLYPHEASP
6   VALVALTHRLEUSERGLYTHRARGGLYPRO
7   LEUSERARGVALGLYLEUGLUPROPROPRO
8   GLYLYSARGGLUCYSARGVALGLYGLNTYR
9   VALVALASPLEUTHRSERPHEGLUGLNLEU
10   ALALEUPROVALLEUARGASNALAASPCYS
11   SERSERGLYPROGLYGLNARGVALCYSVAL
12   ILEASPGLUILEGLYLYSMETGLULEUPHE
13   SERGLNLEUPHEILEGLNALAVALARGGLN
14   THRLEUSERTHRPROGLYTHRILEILELEU
15   GLYTHRILEPROVALPROLYSGLYLYSPRO
16   LEUALALEUVALGLUGLUILEARGASNARG
17   LYSASPVALLYSVALPHEASNVALTHRLYS
18   GLUASNARGASNHISLEULEUPROASPILE
19   VALTHRCYSVALGLNSERSERARGLYS

Entity 2, Magnesium ion - Mg - 24.305 Da.

1   MG

Samples:

sample_1: 32324, [U-13C; U-15N], 2 mM; MES, [U-2H], 25 mM; DTT, [U-2H], 5 mM; MgCl2 10 mM

conditions_1: pH: 6.4; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
2D 15N1H HSQCsample_1not availableconditions_1
3D HNCAsample_1not availableconditions_1
3D HNCACBsample_1not availableconditions_1
3D CBCA(CO)NHsample_1not availableconditions_1
3D HC(C)H-TOCSYsample_1not availableconditions_1
3D H(C)C(CO)NHsample_1not availableconditions_1
2D 13C1H HSQCsample_1not availableconditions_1
3D 15N [1H,1H]-TOCSYsample_1not availableconditions_1
3D 13C [1H,1H]-NOESY aliphaticsample_1not availableconditions_1
3D 15N [1H,1H}-NOESYsample_1not availableconditions_1
3D HNCOsample_1not availableconditions_1
3D HNHAsample_1not availableconditions_1
3D HNHAHBsample_1not availableconditions_1
3D 13C [1H,1H]-NOESY aromaticsample_1not availableconditions_1

Software:

CARA v1.5.3, CARA - Spectral analysis, data management

Topspin v1.3.b.16, Bruker Biospin - processing, spectral acquisition

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks