BMRB Entry 7116

Title:
The solution structure of PHS018 from pyrococcus horikoshii
Deposition date:
2006-05-17
Original release date:
2006-11-13
Authors:
Coles, M.; Hulko, M.; Truffault, V.; Martin, J.; Lupas, A.
Citation:

Citation: Coles, M.; Hulko, M.; Djuranovic, S.; Truffault, V.; Koretke, K.; Martin, J.; Lupas, A.. "Common evolutionary origin of swapped-hairpin and double-psi beta barrels"  Structure 14, 1489-1498 (2006).
PubMed: 17027498

Assembly members:

Assembly members:
PhS018, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pyrococcus horikoshii   Taxonomy ID: 53953   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus horikoshii

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts420
15N chemical shifts89
1H chemical shifts700
coupling constants83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
192aa long hypothetical protein1

Entities:

Entity 1, 92aa long hypothetical protein 92 residues - Formula weight is not available

1   METASPVALLEUALALYSPHEHISTHRTHR
2   VALHISARGILEGLYARGILEILEILEPRO
3   ALAGLYTHRARGLYSPHETYRGLYILEGLU
4   GLNGLYASPPHEVALGLUILELYSILEVAL
5   LYSTYRGLUGLYGLUGLUPROLYSGLUGLY
6   THRPHETHRALAARGVALGLYGLUGLNGLY
7   SERVALILEILEPROLYSALALEUARGASP
8   VALILEGLYILELYSPROGLYGLUVALILE
9   GLUVALLEULEULEUGLYHISTYRLYSPRO
10   ARGASN

Samples:

sample_1: PhS018 0.5 mM; PHOSPHATE BUFFER 50 mM; NaCL 50 mM; H2O 90%; D2O 10%

sample_2: PhS018, [U-15N], 0.5 mM; PHOSPHATE BUFFER 50 mM; NaCL 50 mM; H2O 90%; D2O 10%

sample_3: PhS018, [U-13C; U-15N], 0.5 mM; PHOSPHATE BUFFER 50 mM; NaCL 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 80 mM; pH: 3.0; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CCH-COSYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available

Software:

X-PLOR vNIH-2.9.7 - refinement, structure solution

xwinnmr v3.5 - processing

NMRView v5.0.15 - data analysis

NMR spectrometers:

  • BRUKER DMX 900 MHz
  • BRUKER DMX 600 MHz

Related Database Links:

PDB
DBJ BAA29497
REF NP_142395 WP_010884522

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks