BMRB Entry 7082

Title:
1H, 13C, and 15N assignments for the Psuedomonas putida protein PutA45
Deposition date:
2006-04-21
Original release date:
2009-05-27
Authors:
Halouska, Steven; Zhou, Yuzhen; Becker, Donald; Powers, Robert
Citation:

Citation: Halouska, Steven; Zhou, Yuzhen; Becker, Donald; Powers, Robert. "Solution Structure of the Pseudomonas putida protein PpPutA45 and its DNA complex"  Proteins 75, 12-27 (2009).
PubMed: 18767154

Assembly members:

Assembly members:
PutA45, polymer, 51 residues, 6007 Da.

Natural source:

Natural source:   Common Name: Psuedomonas putida   Taxonomy ID: 303   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Psuedomonas putida

Experimental source:

Experimental source:   Production method: Purified from the natural source

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts200
15N chemical shifts42
1H chemical shifts316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PutA45 unit11
2PutA45 unit21

Entities:

Entity 1, PutA45 unit1 51 residues - 6007 Da.

1   METALATHRTHRTHRLEUGLYVALLYSLEU
2   ASPASPPROTHRARGGLUARGLEULYSALA
3   ALAALAGLNSERILEASPARGTHRPROHIS
4   TRPLEUILELYSGLNALAILEPHEASNTYR
5   LEUGLULYSLEUGLUHISHISHISHISHIS
6   HIS

Samples:

sample_1: PutA45, [U-99% 13C, U-99% 15 N], 2.2 mM; phospate buffer 50 mM; NaCl 200 mM; D2O 10%; H2O 90%

conditions_1: ionic strength: 200 mM; pH: 6.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
CBCANHsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HCCCONHsample_1not availableconditions_1
CCCONHsample_1not availableconditions_1
C13-NOESYsample_1not availableconditions_1
N15-NOESYsample_1not availableconditions_1
HNHAsample_1not availableconditions_1

Software:

NMRPipe v2.2 -

PIPP v4.3.5 -

AutoAssign v1.11.1 -

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAN56864 BAO59856
EMBL CAK17638 CAY46727 CDF97094
GB AAF25000 AAF73193 AAN70514 AAO58445 AAY95904
REF NP_747050 NP_794750 WP_002551755 WP_003171271 WP_003186541

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks