BMRB Entry 7014

Title:
NMR assigment of the SARS-CoV protein nsp1   PubMed: 16821128
Deposition date:
2006-03-06
Original release date:
2006-08-09
Authors:
Almeida, Marcius; Geralt, Michael; Johnson, Margaret; Saikatendu, Kumar; Joseph, Jeremiah; Subramanian, Vanitha; Stevens, Raymond; Kuhn, Peter; Wuthrich, Kurt
Citation:

Citation: Almeida, Marcius; Johnson, Margaret; Wuthrich, Kurt. "NMR assignment of the SARS-CoV protein nsp1"  J. Biomol. NMR 36, 46-46 (2006).

Assembly members:

Assembly members:
leader protein from SARS-CoV, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV   Taxonomy ID: 227859   Superkingdom: Viruses   Kingdom: not available   Genus/species: Coronavirus SARS coronavirus

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: SARS-CoV   Taxonomy ID: 227859   Superkingdom: Viruses   Kingdom: not available   Genus/species: Coronavirus SARS coronavirus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts503
15N chemical shifts116
1H chemical shifts833

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nsp11

Entities:

Entity 1, nsp1 116 residues - Formula weight is not available

1   GLYHISVALGLNLEUSERLEUPROVALLEU
2   GLNVALARGASPVALLEUVALARGGLYPHE
3   GLYASPSERVALGLUGLUALALEUSERGLU
4   ALAARGGLUHISLEULYSASNGLYTHRCYS
5   GLYLEUVALGLULEUGLULYSGLYVALLEU
6   PROGLNLEUGLUGLNPROTYRVALPHEILE
7   LYSARGSERASPALALEUSERTHRASNHIS
8   GLYHISLYSVALVALGLULEUVALALAGLU
9   METASPGLYILEGLNTYRGLYARGSERGLY
10   ILETHRLEUGLYVALLEUVALPROHISVAL
11   GLYGLUTHRPROILEALATYRARGASNVAL
12   LEULEUARGLYSASNGLY

Samples:

sample_1: leader protein from SARS-CoV, [U-13C; U-15N], 2 ± 0.2 mM; Na Phosphate 25 mM; NaCl 250 mM; Azide 0.03%; D2O 10%

conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D [15N,1H]-HSQCsample_1not availableconditions_1
3D HNCAsample_1not availableconditions_1
2D [13C,1H]-HSQCsample_1not availableconditions_1
3D HNCOsample_1not availableconditions_1
3D HNCACBsample_1not availableconditions_1
3D CBCA(CO)NHsample_1not availableconditions_1
3D HBHA(CO)NHsample_1not availableconditions_1
3D CC(co)NHsample_1not availableconditions_1
3D 13C-[1H,1H] NOESYsample_1not availableconditions_1
3D 15N-[1H,1H] NOESYsample_1not availableconditions_1

Software:

CARA v1.4, . - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAC81346 BAC81347 BAC81360 BAC81361 BAC81374
GB AAP13439 AAP13442 AAP13566 AAP13575 AAP30028
REF NP_828849 NP_828850 NP_828860
SP P0C6F8 P0C6U8 P0C6W2 P0C6X7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks