BMRB Entry 7013

Title:
1H, 15N, 13C resonance assignments for Bcl-xL protein 38 kDa dimer
Deposition date:
2006-03-01
Original release date:
2006-03-31
Authors:
Denisov, Alexey; Gehring, Kalle
Citation:

Citation: Denisov, Alexey; Sprules, Tara; Fraser, James; Kozlov, Guennadi; Gehring, Kalle. "Heat-induced dimerization of Bcl-xL through alpha-helix swapping"  Biochemistry 46, 734-740 (2007).
PubMed: 17223694

Assembly members:

Assembly members:
Bcl-xL, polymer, 163 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET-29b

Data sets:
Data typeCount
13C chemical shifts432
15N chemical shifts151
1H chemical shifts208

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bcl-xL, chain 11
2Bcl-xL, chain 21

Entities:

Entity 1, Bcl-xL, chain 1 163 residues - Formula weight is not available

Protein contains C-terminal His-tag LEHHHHHH

1   SERGLNSERASNARGGLULEUVALVALASP
2   PHELEUSERTYRLYSLEUSERGLNARGGLY
3   TYRSERTRPSERGLNPHESERASPVALGLU
4   GLUASNARGTHRGLUALAPROGLUGLUTHR
5   GLUALAGLUALAVALLYSGLNALALEUARG
6   GLUALAGLYASPGLUPHEGLULEUARGTYR
7   ARGARGALAPHESERASPLEUTHRSERGLN
8   LEUHISILETHRPROGLYTHRALATYRGLN
9   SERPHEGLUGLNVALVALASNGLULEUPHE
10   ARGASPGLYVALASNTRPGLYARGILEVAL
11   ALAPHEPHESERPHEGLYGLYALALEUCYS
12   VALGLUSERVALASPLYSGLUMETGLNVAL
13   LEUVALSERARGILEALASERTRPMETALA
14   THRTYRLEUASNASPHISLEUGLUPROTRP
15   ILEGLNGLUASNGLYGLYTRPASPTHRPHE
16   VALASPLEUTYRGLYLEUGLUHISHISHIS
17   HISHISHIS

Samples:

sample_1: Bcl-xL dimer, [U-99% 13C; U-99% 15N; U-80% 2D], 1 mM; phosphate buffer 25 mM; D2O 10%; H2O 90%

conditions_1: pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H15N-TROSY-HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
3D-NOESY-HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1

Software:

XEASY v1.3.13 - processing

NMR spectrometers:

  • Varian Unity Inova 800 MHz

Related Database Links:

DBJ BAE87681

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks