BMRB Entry 7004

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Rhodopseudomonas palustrus protein Rpa0253. Northeast structural genomics target RpR3.
Deposition date:
2006-02-23
Original release date:
2006-03-29
Authors:
Ramelot, Theresa; Kennedy, Michael
Citation:

Citation: Ramelot, Theresa; Kennedy, Michael. "NMR structure of Rpa0253 from Rhodopseudomonas paulstris"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
Rpa0253, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rhodopseudomonas palustris   Taxonomy ID: 1076   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Rhodopseudomonas palustris

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET21

Data sets:
Data typeCount
13C chemical shifts625
15N chemical shifts150
1H chemical shifts1014
coupling constants77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rpa0253 monomer1

Entities:

Entity 1, Rpa0253 monomer 145 residues - Formula weight is not available

1   VALALATYRTRPLEUVALLYSSERGLUPRO
2   SERVALTRPSERTRPASPGLNGLNVALALA
3   LYSGLYALAALAGLYGLUALATRPTHRGLY
4   VALARGASNHISSERALALYSLEUHISMET
5   VALALAMETARGARGGLYASPARGALAPHE
6   TYRTYRHISSERASNGLUGLYLYSGLUILE
7   VALGLYILEALAGLUILEILEARGGLUALA
8   TYRPROASPPROTHRASPALASERGLYLYS
9   PHEVALCYSVALASPILELYSALAASPLYS
10   PROLEULYSTHRPROVALTHRLEUALAALA
11   VALLYSALAGLUPROARGLEUALAASPMET
12   ALALEUMETLYSTYRSERARGLEUSERVAL
13   GLNPROVALTHRALAGLUGLUTRPLYSLEU
14   VALCYSLYSMETGLYGLYLEULEUGLUHIS
15   HISHISHISHISHIS

Samples:

sample_1: Rpa0253, [U-100% 13C; U-100% 15N], 0.5 mM; MES 20 mM; NaCl 5 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 5%

conditions_1: ionic strength: 100 mM; pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 799.7 MHz
  • Varian Inova 600.2 MHz

Related Database Links:

PDB
EMBL CAE25697
GB ACE98814 ADU42022
REF NP_945606 WP_011155821 WP_012494016 WP_013500221 YP_001989290

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks