BMRB Entry 6984

Title:
1H, 13C, and 15N Chemical Shift Assignments of the cAMP-Binding Domain A of the PKA Regulatory Subunit
Deposition date:
2006-02-14
Original release date:
2006-12-22
Authors:
Esposito, Veronica; Sjoberg, Tim; Das, Rahul; Brown, Simon; Taylor, Susan; Melacini, Giuseppe
Citation:

Citation: Esposito, Veronica; Sjoberg, Tim; Das, Rahul; Brown, Simon; Taylor, Susan; Melacini, Giuseppe. "NMR assignment of the cAMP-binding domain A of the PKA regulatory subunit"  J. Biomol. NMR 36, 64-64 (2006).
PubMed: 17016670

Assembly members:

Assembly members:
PKA regulatory subunit, polymer, 126 residues, 14156 Da.
CMP, non-polymer, 329.206 Da.

Natural source:

Natural source:   Common Name: Bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos Taurus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts476
15N chemical shifts130
1H chemical shifts790

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cAMP Binding Domain A1
2cAMP2

Entities:

Entity 1, cAMP Binding Domain A 126 residues - 14156 Da.

1   ASPTYRLYSTHRMETALAALALEUALALYS
2   ALAILEGLULYSASNVALLEUPHESERHIS
3   LEUASPASPASNGLUARGSERASPILEPHE
4   ASPALAMETPHEPROVALSERPHEILEALA
5   GLYGLUTHRVALILEGLNGLNGLYASPGLU
6   GLYASPASNPHETYRVALILEASPGLNGLY
7   GLUMETASPVALTYRVALASNASNGLUTRP
8   ALATHRSERVALGLYGLUGLYGLYSERPHE
9   GLYGLULEUALALEUILETYRGLYTHRPRO
10   ARGALAALATHRVALLYSALALYSTHRASN
11   VALLYSLEUTRPGLYILEASPARGASPSER
12   TYRARGARGILELEUMETGLYSERTHRLEU
13   ARGLYSARGLYSMETTYR

Entity 2, cAMP - C10 H12 N5 O6 P - 329.206 Da.

1   CMP

Samples:

sample_1: R1a 119-244, [U-13C; U-15N], 0.5 mM; cAMP 1 mM; KH2PO4 20 mM; NaCl 100 mM; 1:1 mixture of Arg:Glu 50 mM; D2O 5%

conditions_1: pH: 6.5; temperature: 309 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HBHANHsample_1not availableconditions_1
HBHA(CO)NHsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
15N TOCSY-HSQCsample_1not availableconditions_1
15N NOESY-HSQCsample_1not availableconditions_1
15N HSQC-NOESY-15N HSQCsample_1not availableconditions_1
13C HSQC-NOESY-15N HSQCsample_1not availableconditions_1
HCcH-COSYsample_1not availableconditions_1
HCcH-TOCSYsample_1not availableconditions_1
hCCG-TOCSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAA60266 AAF00035 AEH77255 AEH77256 AEH77257

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks