BMRB Entry 6976

Title:
Solution NMR structure of the UPF0346 protein yozE from Bacillus subtilis. Northeast Structural Genomics target SR391.
Deposition date:
2006-02-09
Original release date:
2006-04-06
Authors:
Rossi, P.
Citation:

Citation: Rossi, P.; Acton, T.; Cunningham, K.; Ma, L.; Shetty, K.; Swapna, G.; Xiao, R.; Montelione, G.. "Solution NMR structure of the UPF0346 protein yozE from Bacillus subtilis. Northeast Structural Genomics target SR391."  .

Assembly members:

Assembly members:
Hypothetical UPF0346 protein yozE, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hypothetical UPF0346 protein yozE: MKSFYHYLLKYRHPKPKDSI SEFANQAYEDHSFPKTSTDY HEISSYLELNADYLHTMATF DEAWDQYESEVHGRLEHHHH HH

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts80
1H chemical shifts513

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hypothetical UPF0346 protein yozE1

Entities:

Entity 1, Hypothetical UPF0346 protein yozE 82 residues - Formula weight is not available

1   METLYSSERPHETYRHISTYRLEULEULYS
2   TYRARGHISPROLYSPROLYSASPSERILE
3   SERGLUPHEALAASNGLNALATYRGLUASP
4   HISSERPHEPROLYSTHRSERTHRASPTYR
5   HISGLUILESERSERTYRLEUGLULEUASN
6   ALAASPTYRLEUHISTHRMETALATHRPHE
7   ASPGLUALATRPASPGLNTYRGLUSERGLU
8   VALHISGLYARGLEUGLUHISHISHISHIS
9   HISHIS

Samples:

sample_1: Hypothetical UPF0346 protein yozE, [U-13C; U-15N], 1.5 mM; CaCl2 5 mM; NaCl 100 mM; NH4OAc 20 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_2: Hypothetical UPF0346 protein yozE, [U-5% 13C; U-15N], 1.35 mM; CaCl2 5 mM; NaCl 100 mM; NH4OAc 20 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_cond_1: ionic strength: 100 mM; pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1
HNCA-Jnot availablenot availablesample_cond_1
HiRes-CH HSQC for stereospec. VL Methyl assign.not availablenot availablesample_cond_1
HNCACBnot availablenot availablesample_cond_1
HNcoCACBnot availablenot availablesample_cond_1
HNCOnot availablenot availablesample_cond_1
HNCAnot availablenot availablesample_cond_1
CccoNH tocsynot availablenot availablesample_cond_1
HCCH-COSYnot availablenot availablesample_cond_1
HAcacoNH single transfernot availablenot availablesample_cond_1

Software:

VNMR v6.1c - collection

X-PLOR NIH v2.0.6 - refinement

NMRPipe v2.5 - processing

SPARKY v2.11 - data analysis

AutoStruct v2.1.1 - structure solution

AutoAssign v1.17 - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAI85645 BAM52635 BAM58210 GAK79322
EMBL CAB13859 CCU58650 CEI57174 CEJ77599
GB ADV92877 AEP86996 AEP91168 AFI28688 AFQ57911
REF NP_389849 WP_003231160 WP_003241575 WP_010331315 WP_014114115
SP O31864
AlphaFold O31864

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks