BMRB Entry 6969

Title:
Chemical Shift Assignment for SARS-CoV S2-HR2   PubMed: 16507566
Deposition date:
2006-02-06
Original release date:
2006-04-27
Authors:
Hakansson-McReynolds, Susanna; Caffrey, Michael; Jiang, Shaokai
Citation:

Citation: Hakansson-McReynolds, Susanna; Jiang, Shaokai; Rong, Lijun; Caffrey, Michael. "Solution structure of the SARS-coronavirus HR2 domain in the prefusion state"  J. Biol. Chem. 281, 11965-11971 (2006).

Assembly members:

Assembly members:
S2-Hr2, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS coronavirus   Taxonomy ID: 227859   Superkingdom: Viruses   Kingdom: not available   Genus/species: Coronavirus SARS coronavirus

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: SARS coronavirus   Taxonomy ID: 227859   Superkingdom: Viruses   Kingdom: not available   Genus/species: Coronavirus SARS coronavirus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts213
15N chemical shifts58
1H chemical shifts333

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S2-HR2, chain 11
2S2-HR2, chain 21
3S2-HR2, chain 31

Entities:

Entity 1, S2-HR2, chain 1 55 residues - Formula weight is not available

1   GLYSERHISTHRSERPROASPVALASPLEU
2   GLYASPILESERGLYILEASNALASERVAL
3   VALASNILEGLNLYSGLUILEASPARGLEU
4   ASNGLUVALALALYSASNLEUASNGLUSER
5   LEUILEASPLEUGLNGLULEUGLYLYSTYR
6   GLUGLNTYRILELYS

Samples:

sample_1: S2-Hr2, [U-95% 13C; U-95% 15N], 1 ± 0.1 mM; TFE 30%

conditions_1: pH: 7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
HNC0sample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
CBCANHsample_1not availableconditions_1
15N-edited TOCSY-HSQCsample_1not availableconditions_1
HCC(CO)NHsample_1not availableconditions_1
CC(CO)NHsample_1not availableconditions_1
1H15N NOESY-HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAC81348 BAC81362 BAC81376 BAC81390 BAC81404
GB AAP13441 AAP13567 AAP30030 AAP30713 AAP37017
REF NP_828851
SP P59594 Q0Q475 Q3I5J5 Q3LZX1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks