BMRB Entry 6966

Title:
1H and 15N assignment of cytochrome c552 from Thermus thermophilus in the reduced state
Deposition date:
2006-02-02
Original release date:
2006-04-27
Authors:
Muresanu, Lucia; Pristovsek, Primoz; Loehr, Frank; Maneg, Oliver; Mukrasch, Marco; Rueterjans, Heinz; Ludwig, Bernd; Luecke, Christian
Citation:

Citation: Muresanu, Lucia; Pristovsek, Primoz; Loehr, Frank; Maneg, Oliver; Mukrasch, Marco; Rueterjans, Heinz; Ludwig, Bernd; Luecke, Christian. "The electron transfer complex between cytochrome c552 and the CuA domain of the Thermus thermophilus ba3 oxidase - a combined NMR and computational approach"  J. Biol. Chem. 281, 14503-14513 (2006).
PubMed: 16554303

Assembly members:

Assembly members:
cytochrome c552, polymer, 133 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET-22b

Data sets:
Data typeCount
15N chemical shifts144
1H chemical shifts998

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cyt c5521
2heme c2

Entities:

Entity 1, cyt c552 133 residues - Formula weight is not available

The first 2 residues (Met1 and Ala2) are recombinant artifacts.

1   METALAGLNALAASPGLYALALYSILETYR
2   ALAGLNCYSALAGLYCYSHISGLNGLNASN
3   GLYGLNGLYILEPROGLYALAPHEPROPRO
4   LEUALAGLYHISVALALAGLUILELEUALA
5   LYSGLUGLYGLYARGGLUTYRLEUILELEU
6   VALLEULEUTYRGLYLEUGLNGLYGLNILE
7   GLUVALLYSGLYMETLYSTYRASNGLYVAL
8   METSERSERPHEALAGLNLEULYSASPGLU
9   GLUILEALAALAVALLEUASNHISILEALA
10   THRALATRPGLYASPALALYSLYSVALLYS
11   GLYPHELYSPROPHETHRALAGLUGLUVAL
12   LYSLYSLEUARGALALYSLYSLEUTHRPRO
13   GLNGLNVALLEUTHRGLUARGLYSLYSLEU
14   GLYLEULYS

Entity 2, heme c - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: cytochrome c552 2 mM; potassium phosphate 20 mM; sodium ascorbate 5 mM

sample_2: cytochrome c552, [U-98% 15N], 2 mM; potassium phosphate 20 mM; sodium ascorbate 5 mM

conditions_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H_TOCSYnot availablenot availableconditions_1
1H-1H_NOESYnot availablenot availableconditions_1
15N-1H_TROSYnot availablenot availableconditions_1
15N-1H_TOCSY-TROSYnot availablenot availableconditions_1
15N-1H_NOESY-TROSYnot availablenot availableconditions_1

Software:

xwinnmr v3.5, Bruker - data collection and processing

AURELIA v2.5.9, Bruker - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks