BMRB Entry 6873

Title:
1H, 15N and 13C chemical shift assignments of pleckstrin's C-terminal PH domain
Deposition date:
2005-10-25
Original release date:
2006-05-02
Authors:
Edlich, Christian; Simon, Bernd; Stier, Gunter; Sattler, Michael; Muhle-Goll, Claudia
Citation:

Citation: Edlich, Christian; Simon, Bernd; Sattler, Michael; Muhle-Goll, Claudia. "New NMR Assignment 1H, 13C, and 15N Assignment of the Second PH Domain of Human Pleckstrin (234-350)"  J. Biomol. NMR 36, 21-21 (2006).
PubMed: 16642402

Assembly members:

Assembly members:
C-PH, polymer, 360 residues, 13455.3 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts401
15N chemical shifts120
1H chemical shifts790

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pleckstrin C-PH1

Entities:

Entity 1, pleckstrin C-PH 360 residues - 13455.3 Da.

assignment starting from residue 234 to 350.

1   METHISHISHISHISHISHISLYSPROMET
2   METGLUPROLYSARGILEARGGLUGLYTYR
3   LEUVALLYSLYSGLYSERVALPHEASNTHR
4   TRPLYSPROMETTRPVALVALLEULEUGLU
5   ASPGLYILEGLUPHETYRLYSLYSLYSSER
6   ASPASNSERPROLYSGLYMETILEPROLEU
7   LYSGLYSERTHRLEUTHRSERPROCYSGLN
8   ASPPHEGLYLYSARGMETPHEVALPHELYS
9   ILETHRTHRTHRLYSGLNGLNASPHISPHE
10   PHEGLNALAALAPHELEUGLUGLUARGASP
11   ALATRPVALARGASPILEASNLYSALAILE
12   LYSCYSILEGLUGLYGLYGLNLYSPHEALA
13   ARGLYSSERTHRARGARGSERILEARGLEU
14   PROGLUTHRILEASPLEUGLYALALEUTYR
15   LEUSERMETLYSASPTHRGLULYSGLYILE
16   LYSGLULEUASNLEUGLULYSASPLYSLYS
17   ILEPHEASNHISCYSPHETHRGLYASNCYS
18   VALILEASPTRPLEUVALSERASNGLNSER
19   VALARGASNARGGLNGLUGLYLEUMETILE
20   ALASERSERLEULEUASNGLUGLYTYRLEU
21   GLNPROALAGLYASPMETSERLYSSERALA
22   VALASPGLYTHRALAGLUASNPROPHELEU
23   ASPASNPROASPALAPHETYRTYRPHEPRO
24   ASPSERGLYPHEPHECYSGLUGLUASNSER
25   SERASPASPASPVALILELEULYSGLUGLU
26   PHEARGGLYVALILEILELYSGLNGLYCYS
27   LEULEULYSGLNGLYHISARGARGLYSASN
28   TRPLYSVALARGLYSPHEILELEUARGGLU
29   ASPPROALATYRLEUHISTYRTYRASPPRO
30   ALAGLYALAGLUASPPROLEUGLYALAILE
31   HISLEUARGGLYCYSVALVALTHRSERVAL
32   GLUSERASNSERASNGLYARGLYSSERGLU
33   GLUGLUASNLEUPHEGLUILEILETHRALA
34   ASPGLUVALHISTYRPHELEUGLNALAALA
35   THRPROLYSGLUARGTHRGLUTRPILELYS
36   ALAILEGLNMETALASERARGTHRGLYLYS

Samples:

sample_1: Pleckstrin, [U-2H; U-13C; U-15N], 0.4 mM; sodium phosphate buffer 20 mM; NaCl 100 mM; DTT 2 mM; sodium azide 0.02%

conditions_1: ionic strength: 100 mM; pH: 6.8; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
1H13C HSQCsample_1not availableconditions_1
Triple-resonance experimentssample_1not availableconditions_1
HCCH tocsysample_1not availableconditions_1
13C-HMQC-NOESYsample_1not availableconditions_1
15N-HSQC-NOESYsample_1not availableconditions_1

Software:

NMRView -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

DBJ BAG36495 BAI47103
EMBL CAA30564 CAG46853 CAG46876
GB AAH18549 AAP36299 AAX37008 AAX43402 AAX93121
PRF 1408254A
REF NP_002655 XP_001094492 XP_002811995 XP_003262529 XP_003830964
SP P08567
AlphaFold P08567

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks