BMRB Entry 6845

Title:
NMR Structural analysis of archaeal Nop10
Deposition date:
2005-09-29
Original release date:
2005-12-28
Authors:
Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.
Citation:

Citation: Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.. "The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs"  Nat. Struct. Mol. Biol. 12, 1101-1107 (2005).
PubMed: 16286935

Assembly members:

Assembly members:
Ribosome biogenesis protein Nop10, polymer, 63 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Methanocaldococcus jannaschii   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanocaldococcus jannaschii

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ribosome biogenesis protein Nop10: GSHMVEMRMKKCPKCGLYTL KEICPKCGEKTVIPKPPKFS LEDRWGKYRRMLKRALKNKN KAE

Data sets:
Data typeCount
13C chemical shifts273
15N chemical shifts64
1H chemical shifts480

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ribosome biogenesis protein Nop101
2ZINC (II) ION2

Entities:

Entity 1, Ribosome biogenesis protein Nop10 63 residues - Formula weight is not available

1   GLYSERHISMETVALGLUMETARGMETLYS
2   LYSCYSPROLYSCYSGLYLEUTYRTHRLEU
3   LYSGLUILECYSPROLYSCYSGLYGLULYS
4   THRVALILEPROLYSPROPROLYSPHESER
5   LEUGLUASPARGTRPGLYLYSTYRARGARG
6   METLEULYSARGALALEULYSASNLYSASN
7   LYSALAGLU

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Ribosome biogenesis protein Nop10 1 mM; Sodium Acetate 50 mM; KCl 100 mM; DTT 2 mM; ZnCl2 100 uM; H2O 90%; D2O 10%

sample_2: Ribosome biogenesis protein Nop10 1 mM; Sodium Acetate 50 mM; KCl 100 mM; DTT 2 mM; ZnCl2 100 uM; D2O 100%

sample_3: Ribosome biogenesis protein Nop10, [U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; DTT 2 mM; ZnCl2 100 uM; H2O 90%; D2O 10%

sample_4: Ribosome biogenesis protein Nop10, [U-13C; U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; DTT 2 mM; ZnCl2 100 uM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1

Software:

CYANA vv. 2.0, Guntert, P. - refinement

NMRPipe vv. 2.3, Delaglio, F. - processing

SPARKY vv. 3.110, Goddard. T.D. - data analysis

TALOS vv. 2003.027.13.05, Cornilescu, G. - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AAB98109
REF NP_247081 WP_010869609
SP P81303
AlphaFold P81303

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks