BMRB Entry 6833

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for holo-MazF(E24A) with MazEp(54-77)
Deposition date:
2005-09-21
Original release date:
2007-01-29
Authors:
Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko
Citation:

Citation: Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko. "Characterization of Dual Substrate Binding Sites in the Homodimeric Structure of Escherichia coli mRNA Interferase MazF"  J. Mol. Biol. 357, 139-150 (2006).
PubMed: 16413577

Assembly members:

Assembly members:
E. COLI TOXIN mRNA interferase, polymer, 114 residues, 12321 Da.
MazEp(54-77), polymer, 24 residues, 2890 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: Protista   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts212
15N chemical shifts101
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MazF(E24A)1
2MazF(E24A)1
3MazEp(54-77)2
4MazEp(54-77)2

Entities:

Entity 1, MazF(E24A) 114 residues - 12321 Da.

1   GLYSERHISMETVALSERARGTYRVALPRO
2   ASPMETGLYASPLEUILETRPVALASPPHE
3   ASPPROTHRLYSGLYSERALAGLNALAGLY
4   HISARGPROALAVALVALLEUSERPROPHE
5   METTYRASNASNLYSTHRGLYMETCYSLEU
6   CYSVALPROCYSTHRTHRGLNSERLYSGLY
7   TYRPROPHEGLUVALVALLEUSERGLYGLN
8   GLUARGASPGLYVALALALEUALAASPGLN
9   VALLYSSERILEALATRPARGALAARGGLY
10   ALATHRLYSLYSGLYTHRVALALAPROGLU
11   GLULEUGLNLEUILELYSALALYSILEASN
12   VALLEUILEGLY

Entity 2, MazEp(54-77) 24 residues - 2890 Da.

1   THRLEUALAGLULEUVALASNASPILETHR
2   PROGLUASNLEUHISGLUASNILEASPTRP
3   GLYGLUPROLYS

Samples:

sample_1: MazF(E24A) mutant, [U-95% 13C; U-95% 15N], 0.8 ± 0.1 mM; MazEp(54-77) 1.5 ± 0.1 mM; NaPi 20 mM; NaCl 100 mM; DTT 5 mM; TCEP 2 mM; NaN3 1 mM; AEBSF 1 mM; D2O 10%; H2O 90%

conditions_1: pH: 7.00; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
CBCACONNHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1

Software:

XEASY v1.3 -

NMR spectrometers:

  • Varian Unity-Inova 600 MHz

Related Database Links:

PDB
DBJ BAA41177 BAB37066 BAE76857 BAG78565 BAI27044
EMBL CAP77216 CAQ33107 CAQ87849 CAQ99711 CAR04293
GB AAA03238 AAA69293 AAC75825 AAG57896 AAZ89539
REF NP_289337 NP_311670 NP_417263 WP_000581935 WP_000581937
SP P0AE72 P0AE73
AlphaFold P0AE72 P0AE73

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks