BMRB Entry 6826

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for N-terminal DNA recognition domain of the Bacillus subtilis of the transcription-state regulator Abh
Deposition date:
2005-09-14
Original release date:
2006-06-28
Authors:
Bobay, Benjamin; Muller, Geoffery; Thompson, Richele; Venters, Ronald; Strauch, Mark; Cavanagh, John
Citation:

Citation: Bobay, Benjamin; Mueller, Geoffery; Thompson, Richele; Murzin, Alexey; Venters, Ronald; Strauch, Mark; Cavanagh, John. "NMR structure of AbhN and comparison with AbrBN: First insights into the DNA-binding promiscuity and specificity of AbrB-like transition-state regulator proteins"  J. Biol. Chem. 281, 21399-21409 (2006).
PubMed: 16702211

Assembly members:

Assembly members:
AbhN, polymer, 54 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus Subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: PET21-b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts219
15N chemical shifts50
1H chemical shifts353

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AbhN subunit 11
2AbhN subunit 21

Entities:

Entity 1, AbhN subunit 1 54 residues - Formula weight is not available

1   METLYSSERILEGLYVALVALARGLYSVAL
2   ASPGLULEUGLYARGILEVALMETPROILE
3   GLULEUARGARGALALEUASPILEALAILE
4   LYSASPSERILEGLUPHEPHEVALASPGLY
5   ASPLYSILEILELEULYSLYSTYRLYSPRO
6   HISGLYVALCYS

Samples:

sample_1: AbhN, [U-95% 13C; U-90% 15N], 1.5 ± 0.2 mM; K-PO 20 ± 0.1 mM; KCl 15 ± 0.1 mM; EDTA 1 ± 0.1 mM; DTT 1 ± 0.1 mM; NaN3 0.02%; D2O 5%; H2O 95%

conditions_1: ionic strength: 15 mM; pH: 5.5; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
H(CCO)NHsample_1not availableconditions_1
C(CO)NHsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA07044 BAA07048 BAM52093 BAM57670 BAO93361
EMBL CAB13321 CCU58013 CEI56628 CEJ77034
GB AAC24923 ADM37537 ADP31955 ADV96466 AEP86424
REF NP_389331 WP_003222317 WP_003232335 WP_003238863 WP_003244728
SP P39758
AlphaFold P39758

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks