BMRB Entry 6800

Title:
NMR solution structure and backbone dynamics of tick-borne E-protein domain III Langat flavivirus
Deposition date:
2005-08-29
Original release date:
2006-11-06
Authors:
Mukherjee, Munia; Dutta, Kaushik; Fox, Robert
Citation:

Citation: Mukherjee, Munia; Dutta, Kaushik; White, Mark; Cowburn, David; Fox, Robert. "NMR solution structure and backbone dynamics of domain III of the E protein of tick-borne Langat flavivirus suggests a potential site for molecular recognition"  Protein Sci. 15, 1342-1355 (2006).
PubMed: 16731969

Assembly members:

Assembly members:
Langat E-D3, polymer, 102 residues, 11164 Da.

Natural source:

Natural source:   Common Name: Flaviviridae   Taxonomy ID: 11085   Superkingdom: viruses   Kingdom: Metazoa   Genus/species: Flavivirus Langat virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: H-MBP-T

Data sets:
Data typeCount
13C chemical shifts383
15N chemical shifts107
1H chemical shifts558

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LGT-E-D3 monomer1

Entities:

Entity 1, LGT-E-D3 monomer 102 residues - 11164 Da.

1   GLYSERGLUPHEGLYSERLYSGLYLEUTHR
2   TYRTHRVALCYSASPLYSTHRLYSPHETHR
3   TRPLYSARGALAPROTHRASPSERGLYHIS
4   ASPTHRVALVALMETGLUVALGLYPHESER
5   GLYTHRARGPROCYSARGILEPROVALARG
6   ALAVALALAHISGLYVALPROGLUVALASN
7   VALALAMETLEUILETHRPROASNPROTHR
8   METGLUASNASNGLYGLYGLYPHEILEGLU
9   METGLNLEUPROPROGLYASPASNILEILE
10   TYRVALGLYASPLEUASNHISGLNTRPPHE
11   GLNLYS

Samples:

sample_1: Langat E-D3, [U-100% 13C; U-100% 15N], 0.7 mM; Tris 10 mM; Bis-Tris 50 mM; NaCL 10 mM

sample_2: Langat E-D3, [U-99% 15N], 0.8 mM

cond_1: ionic strength: 0.1 M; pH: 6.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
C(C0)NHnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
HC(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
N15-NOESY-HSQCnot availablenot availablenot available
N15-TOCSY-HSQCnot availablenot availablenot available
C13-NOESY-HSQCnot availablenot availablenot available

Software:

NMRView v5.04 -

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks