BMRB Entry 6783
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6783
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Clark, Matthew; McElheny, Dan; Wojcik, John; Kurutz, Josh; Rosner, Marsha; Koide, Shohei. "NMR Assignment of Rat Raf Kinase Inhibitor Protein" J. Biomol. NMR 36, 4-4 (2006).
PubMed: 16456706
Assembly members:
Raf Kinase Inhibitor Protein, polymer, 194 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Raf Kinase Inhibitor Protein: GSPGIRLMAADISQWAGPLS
LQEVDEPPQHALRVDYGGVT
VDELGKVLTPTQVMNRPSSI
SWDGLDPGKLYTLVLTDPDA
PSRKDPKFREWHHFLVVNMK
GNDISSGTVLSEYVGSGPPK
DTGLHRYVWLVYEQEQPLNC
DEPILSNKSGDNRGKFKVES
FRKKYHLGAPVAGTCFQAEW
DDSVPKLHDQLAGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 724 |
| 15N chemical shifts | 170 |
| 1H chemical shifts | 909 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RKIP | 1 |
Entities:
Entity 1, RKIP 194 residues - Formula weight is not available
| 1 | GLY | SER | PRO | GLY | ILE | ARG | LEU | MET | ALA | ALA | ||||
| 2 | ASP | ILE | SER | GLN | TRP | ALA | GLY | PRO | LEU | SER | ||||
| 3 | LEU | GLN | GLU | VAL | ASP | GLU | PRO | PRO | GLN | HIS | ||||
| 4 | ALA | LEU | ARG | VAL | ASP | TYR | GLY | GLY | VAL | THR | ||||
| 5 | VAL | ASP | GLU | LEU | GLY | LYS | VAL | LEU | THR | PRO | ||||
| 6 | THR | GLN | VAL | MET | ASN | ARG | PRO | SER | SER | ILE | ||||
| 7 | SER | TRP | ASP | GLY | LEU | ASP | PRO | GLY | LYS | LEU | ||||
| 8 | TYR | THR | LEU | VAL | LEU | THR | ASP | PRO | ASP | ALA | ||||
| 9 | PRO | SER | ARG | LYS | ASP | PRO | LYS | PHE | ARG | GLU | ||||
| 10 | TRP | HIS | HIS | PHE | LEU | VAL | VAL | ASN | MET | LYS | ||||
| 11 | GLY | ASN | ASP | ILE | SER | SER | GLY | THR | VAL | LEU | ||||
| 12 | SER | GLU | TYR | VAL | GLY | SER | GLY | PRO | PRO | LYS | ||||
| 13 | ASP | THR | GLY | LEU | HIS | ARG | TYR | VAL | TRP | LEU | ||||
| 14 | VAL | TYR | GLU | GLN | GLU | GLN | PRO | LEU | ASN | CYS | ||||
| 15 | ASP | GLU | PRO | ILE | LEU | SER | ASN | LYS | SER | GLY | ||||
| 16 | ASP | ASN | ARG | GLY | LYS | PHE | LYS | VAL | GLU | SER | ||||
| 17 | PHE | ARG | LYS | LYS | TYR | HIS | LEU | GLY | ALA | PRO | ||||
| 18 | VAL | ALA | GLY | THR | CYS | PHE | GLN | ALA | GLU | TRP | ||||
| 19 | ASP | ASP | SER | VAL | PRO | LYS | LEU | HIS | ASP | GLN | ||||
| 20 | LEU | ALA | GLY | LYS |
Samples:
sample_1: Raf Kinase Inhibitor Protein, [U-13C; U-15N], 0.7 mM; Tris HCl buffer, [U-2H], 50 mM; NaCl 100 mM; D2O 7%
conditions_1: ionic strength: 120 mM; pH: 7.3; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| C(CCO)NH | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| HA(CO)NH | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA50708 CAA53032 |
| GB | AAH63171 EDM13831 |
| PRF | 2117380A 2207216A |
| REF | NP_058932 |
| SP | P31044 |
| AlphaFold | P31044 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks