BMRB Entry 6746

Title:
Assignment of phnA-like protein rp4479
Deposition date:
2005-07-25
Original release date:
2006-02-23
Authors:
Wu, Bin; Yee, Adelina; Ramelot, Theresa; Semesi, Anthony; Lemak, Alexander; Kennedy, Michael; Arrowsmith, Cheryl
Citation:

Citation: Wu, Bin; Yee, Adelina; Ramelot, Theresa; Semesi, Anthony; Lemak, Alexander; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of phnA-like protein rp4479 from Rhodopseudomonas palustris"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
phnA-like protein rp4479, polymer, 74 residues, 7933 Da.

Natural source:

Natural source:   Common Name: Rhodopseudomonas palustris   Taxonomy ID: 2303   Superkingdom: Bacteria   Kingdom: not available   Genus/species: rhodopseudomonas palustris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET15B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
phnA-like protein rp4479: MSIEVRDCNGALLADGDNVS LIKDLKLKGSSTVLKRGTMI RGIRLTDSEDEIEGRTDKIK GLVLRTEFLKKAGS

Data sets:
Data typeCount
1H chemical shifts520
13C chemical shifts313
15N chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rp44791

Entities:

Entity 1, rp4479 74 residues - 7933 Da.

1   METSERILEGLUVALARGASPCYSASNGLY
2   ALALEULEUALAASPGLYASPASNVALSER
3   LEUILELYSASPLEULYSLEULYSGLYSER
4   SERTHRVALLEULYSARGGLYTHRMETILE
5   ARGGLYILEARGLEUTHRASPSERGLUASP
6   GLUILEGLUGLYARGTHRASPLYSILELYS
7   GLYLEUVALLEUARGTHRGLUPHELEULYS
8   LYSALAGLYSER

Samples:

sample_1: phnA-like protein rp4479, [U-13C; U-15N], 1 mM; NaCl 450 mM; Phosphate 25 mM

cond_set1: pH: 6.5; temperature: 298 K; ionic strength: 0.45 M

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCAcoNHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CCTOCSY_NHnot availablenot availablenot available
HCCTOCSY_NHnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
HCCHTOCSYnot availablenot availablenot available
15N NOESYnot availablenot availablenot available
13C NOESYnot availablenot availablenot available
13,13C 4d CCNOESYnot availablenot availablenot available

Software:

SPARKY v3.95 - spectral analysis, assignemt

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
EMBL CAE29941
GB ACF03482 ADU46252
REF NP_949835 WP_011160033 YP_001993957 YP_004110985

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks