BMRB Entry 6661

Title:
15N hyperfine shifts assignment for rubredoxin (V8A) from Clostridium pasteurianum in the reduced state
Deposition date:
2005-06-08
Original release date:
2005-12-15
Authors:
Lin, I-Jin; Gebel, Erika; Machonkin, Timothy; Westler, William; Markley, John
Citation:

Citation: Lin, I-Jin; Gebel, Erika; Machonkin, Timothy; Westler, William; Markley, John. "Changes in hydrogen-bond strengths explain reduction potentials in 10 rubredoxin variants."  Proc. Natl. Acad. Sci. U. S. A. 11, 14581-14586 (2005).

Assembly members:

Assembly members:
rubredoxin, polymer, 54 residues, Formula weight is not available
FE, non-polymer, 55.845 Da.

Natural source:

Natural source:   Common Name: Clostridium pasteurianum   Taxonomy ID: 1501   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium pasteurianum

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rubredoxin: MKKYTCTACGYIYNPEDGDP DNGVNPGTDFKDIPDDWVCP LCGVGKDQFEEVEE

Data sets:
Data typeCount
15N chemical shifts12

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rubredoxin (V8A)1
2FE (III) ION2

Entities:

Entity 1, rubredoxin (V8A) 54 residues - Formula weight is not available

1   METLYSLYSTYRTHRCYSTHRALACYSGLY
2   TYRILETYRASNPROGLUASPGLYASPPRO
3   ASPASNGLYVALASNPROGLYTHRASPPHE
4   LYSASPILEPROASPASPTRPVALCYSPRO
5   LEUCYSGLYVALGLYLYSASPGLNPHEGLU
6   GLUVALGLUGLU

Entity 2, FE (III) ION - Fe - 55.845 Da.

1   FE

Samples:

reduced_rubredoxin_(V8A): rubredoxin (V8A), [U-15], 4 – 8 mM; phosphate buffer 50 mM

50mM_phosphate_buffer: pH: 6; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1D 15Nreduced_rubredoxin_(V8A)not available50mM_phosphate_buffer

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 4050 4051 4066 4137 4182 4319 4320 5600 6659 6660 6662 6663 6664 6667 6668 6669 6670
PDB
GB AAA23279 AJA49845 AJA53833 ELP57804 KER11884
REF WP_003447684
SP P00268
AlphaFold P00268