BMRB Entry 6576

Title:
Backbone and sidechain 1H assignments of Crotamine
Deposition date:
2005-04-01
Original release date:
2005-10-28
Authors:
Fadel, Valmir; Bettendorff, Pascal; Herrmann, Torsten; de Azevedo, Walter; Oliveira, Eduardo; Yamane, Tetsuo; Wuthrich, Kurt
Citation:

Citation: Fadel, Valmir; Bettendorff, Pascal; Herrmann, Torsten; de Azevedo, Walter; Oliveira, Eduardo; Yamane, Tetsuo; Wuthrich, Kurt. "Automated NMR structure determination and disulfide bond identification of the myotoxin crotamine from Crotalus durissus terrificus"  Toxicon ., .-. (2005).

Assembly members:

Assembly members:
Crotamine, polymer, 42 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: tropical rattlesnake   Taxonomy ID: 8732   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Crotalus durissus terrificus

Experimental source:

Experimental source:   Production method: Purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Crotamine: YKQCHKKGGHCFPKEKICLP PSSDFGKMDCRWRWKCCKKG SG

Data sets:
Data typeCount
1H chemical shifts510
coupling constants22

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Crotamine1

Entities:

Entity 1, Crotamine 42 residues - Formula weight is not available

1   TYRLYSGLNCYSHISLYSLYSGLYGLYHIS
2   CYSPHEPROLYSGLULYSILECYSLEUPRO
3   PROSERSERASPPHEGLYLYSMETASPCYS
4   ARGTRPARGTRPLYSCYSCYSLYSLYSGLY
5   SERGLY

Samples:

D2O_sample: Crotamine, no, 1.8 ± 0.2 mM; D2O 100%

sample: Crotamine, no, 1.8 ± 0.2 mM; D2O 5%; H2O 95%

higher_temperature: pH: 5.8; temperature: 313.4 K

room_temperature: pH: 5.8; temperature: 293.4 K

Experiments:

NameSampleSample stateSample conditions
[1H,_1H]-DQF-COSYnot availablenot availablenot available
[1H,_1H]-TOCSYnot availablenot availablenot available
[1H,_1H]-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
GB AAC02995 AAC06241 AAC19036 AAF34910 AAF34911
PRF 751420A
SP O57540 O73799 P63327 Q9PWF3
AlphaFold P63327 O57540 O73799 Q9PWF3