BMRB Entry 6540

Title:
Sequence-specific 1H, 13C and 15N resonance assignments of the winged-helix domain of the human Werner syndrome protein
Deposition date:
2005-03-07
Original release date:
2005-08-29
Authors:
Sun, Jian-Zhong; Feng, Han-Qiao; Lin, Guang-Xin; Zeng, Wang-Yong; Hu, Jin-Shan
Citation:

Citation: Sun, Jian-Zhong; Feng, Han-Qiao; Lin, Guang-Xin; Zeng, Wangyong; Hu, Jin-Shan. "NMR Assignments of the Winged-Helix Domain of Human Werner Syndrome Protein"  J. Biomol. NMR 32, 261-261 (2005).

Assembly members:

Assembly members:
The winged-helix domain of WRN, polymer, 144 residues, 16333.65 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
1H chemical shifts782
13C chemical shifts604
15N chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1The winged-helix domain of WRN protein1

Entities:

Entity 1, The winged-helix domain of WRN protein 144 residues - 16333.65 Da.

1   METASPASPSERGLUASPTHRSERTRPASP
2   PHEGLYPROGLNALAPHELYSLEULEUSER
3   ALAVALASPILELEUGLYGLULYSPHEGLY
4   ILEGLYLEUPROILELEUPHELEUARGGLY
5   SERASNSERGLNARGLEUALAASPGLNTYR
6   ARGARGHISSERLEUPHEGLYTHRGLYLYS
7   ASPGLNTHRGLUSERTRPTRPLYSALAPHE
8   SERARGGLNLEUILETHRGLYGLYPHELEU
9   VALGLUVALSERARGTYRASNLYSPHEMET
10   LYSILECYSALALEUTHRLYSLYSGLYARG
11   ASNTRPLEUHISLYSALAASNTHRGLUSER
12   GLNSERLEUILELEUGLNALAASNGLUGLU
13   LEUCYSPROLYSLYSLEULEULEUPROSER
14   SERLYSTHRVALSERSERGLYTHRLYSGLU
15   HISCYSTYRASN

Samples:

sample_1: The winged-helix domain of WRN, [U-95% 15N], 0.45 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%

sample_2: The winged-helix domain of WRN, [U-98% 15N], 0.45 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%

sample_3: The winged-helix domain of WRN, [U-95% 13C; U-95% 15N], 0.45 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%

sample_4: The winged-helix domain of WRN 0.29 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%

Ex-cond_1: pH: 7.36; temperature: 295 K; ionic strength: 0.025 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HNCOCOnot availablenot availableEx-cond_1
HNCG_aromnot availablenot availableEx-cond_1
HN(CO)CG_aromnot availablenot availableEx-cond_1
HNCGnot availablenot availableEx-cond_1
HN(CO)Cnot availablenot availableEx-cond_1
HNHAnot availablenot availableEx-cond_1
HBHA(CO)NHnot availablenot availableEx-cond_1
DIPSI-H(CCO)NHnot availablenot availableEx-cond_1
DIPSI-C(CCO)NHnot availablenot availableEx-cond_1
HAHBnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
HCCH-COSYnot availablenot availableEx-cond_1
1H-13C HSQCnot availablenot availableEx-cond_1
15N-HOHAHA-HSQCnot availablenot availableEx-cond_1
15N-NOESY-HSQCnot availablenot availableEx-cond_1
13C-15N NOESYnot availablenot availableEx-cond_1
13C-13C NOESYnot availablenot availableEx-cond_1

Software:

NMRPIPE - data processing, analysis

PIPP - data processing, analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAD92889 BAJ17933
GB AAC41981 AAC63361 AAF06162 AAI72840 AAR05448
REF NP_000544
SP Q14191
AlphaFold Q14191

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks