BMRB Entry 6480

Title:
The loss of stability of a naturally occurring ATP7A mutant is the cause of Menkes disease
Deposition date:
2005-02-02
Original release date:
2005-11-14
Authors:
Banci, L.; Bertini, I.; Cantini, F.; Migliardi, M.; Rosato, A.; Wang, S.
Citation:

Citation: Banci, L.; Bertini, I.; Cantini, F.; Migliardi, M.; Rosato, A.; Wang, S.. "An Atomic-level Investigation of the Disease-causing A629P Mutant of the Menkes Protein, ATP7A."  J. Mol. Biol. 352, 409-417 (2005).
PubMed: 16083905

Assembly members:

Assembly members:
Copper-transporting ATPase 1(E.C.3.6.3.4), polymer, 75 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Copper-transporting ATPase 1(E.C.3.6.3.4): MGDGVLELVVRGMTCASCVH KIESSLTKHRGILYCSVALA TNKAHIKYDPEIIGPRDIIH TIESLGFEASLVKIE

Data sets:
Data typeCount
15N chemical shifts70
1H chemical shifts469

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Copper-transporting ATPase 11
2COPPER (I) ION2

Entities:

Entity 1, Copper-transporting ATPase 1 75 residues - Formula weight is not available

1   METGLYASPGLYVALLEUGLULEUVALVAL
2   ARGGLYMETTHRCYSALASERCYSVALHIS
3   LYSILEGLUSERSERLEUTHRLYSHISARG
4   GLYILELEUTYRCYSSERVALALALEUALA
5   THRASNLYSALAHISILELYSTYRASPPRO
6   GLUILEILEGLYPROARGASPILEILEHIS
7   THRILEGLUSERLEUGLYPHEGLUALASER
8   LEUVALLYSILEGLU

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: Copper-transporting ATPase 1(E.C.3.6.3.4), [U-15N], 0.8 mM; DTT 5 mM; phosphate buffer 100 mM; H2O 90%; D2O 10%

sample_2: Copper-transporting ATPase 1(E.C.3.6.3.4), [U-15N; U-13C], 0.8 mM; DTT 5 mM; phosphate buffer 100 mM; H2O 90%; D2O 10%

sample_3: Copper-transporting ATPase 1(E.C.3.6.3.4) 1.0 mM; DTT 5 mM; phosphate buffer 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
CBCANHnot availablenot availablesample_cond_1
CBCACONHnot availablenot availablesample_cond_1
HNCOnot availablenot availablesample_cond_1
HNCACOnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1

Software:

xwinnmr - collection

CARA v1.2 - data analysis

DYANA v1.5 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
BMRB 6483 6482 6481

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks