BMRB Entry 6375

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6375

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Title:
Full 1H, 13C, and 15N Chemical Shift Assignments for Escherichia coli TonB 151-239
Deposition date:
2004-11-01
Original release date:
2005-03-07
Authors:
Peacock, R.
Citation:

Citation: Sean, Peacock; Weljie, Aalim; Peter, Howard; Price, Feodor; Vogel, Hans. "The solution structure of the C-terminal domain of TonB and interation studies with TonB box peptides."  J. Mol. Biol. 345, 1185-1197 (2005).
PubMed: 15644214

Assembly members:

Assembly members:
TonB C-terminal domain, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TonB C-terminal domain: SGPRALSRNQPQYPARAQAL RIEGQVKVKFDVTPDGRVDN VQILSAKPANMFEREVKNAM RRWRYEPGKPGSGIVVNILF KINGTTEIQ

Data sets:
Data typeCount
1H chemical shifts641
13C chemical shifts359
15N chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TonB C-terminal Domain monomer1

Entities:

Entity 1, TonB C-terminal Domain monomer 89 residues - Formula weight is not available

1   SERGLYPROARGALALEUSERARGASNGLN
2   PROGLNTYRPROALAARGALAGLNALALEU
3   ARGILEGLUGLYGLNVALLYSVALLYSPHE
4   ASPVALTHRPROASPGLYARGVALASPASN
5   VALGLNILELEUSERALALYSPROALAASN
6   METPHEGLUARGGLUVALLYSASNALAMET
7   ARGARGTRPARGTYRGLUPROGLYLYSPRO
8   GLYSERGLYILEVALVALASNILELEUPHE
9   LYSILEASNGLYTHRTHRGLUILEGLN

Samples:

sample_1: TonB C-terminal domain, [U-95% 13C; U-95% 15N], 1.4 mM; Sodium Phosphate 100 mM; Sodium Azide 0.6 mM

conditions_1: pH: 7.00; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D Protonnot availablenot availablenot available
2D Proton-Proton NOESY (H2O)not availablenot availablenot available
2D Proton-Proton TOCSY (D2O)not availablenot availablenot available
2D Proton-Proton NOESY (D2O)not availablenot availablenot available
Steady State 15N Heteronuclear nOenot availablenot availablenot available
T1 15Nnot availablenot availablenot available
T2 15Nnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CCCONH-TOCSYnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
HCCCONH-TOCSYnot availablenot availablenot available
3D 13C separated NOESYnot availablenot availablenot available
3D 15N separated NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAA14784 BAB35175 BAG66413 BAG76825 BAI25062
EMBL CAP75797 CAQ31753 CAQ98131 CAR02647 CAR07605
GB AAB59066 AAB60069 AAB60077 AAB60085 AAB60093
REF NP_287495 NP_309779 NP_415768 NP_707161 NP_753622
SP P02929
AlphaFold P02929

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks