BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6372

Title: 1H and 15N Assigned Chemical Shifts for SARS N-Protein N-Terminate

Deposition date: 2004-10-29 Original release date: 2005-02-10

Authors: Zhong, Nan; Huang, Qing; Xia, Bin

Citation: Zhong, Nan; Huang, Qing; Jin, Changwen; Xia, Bin. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the N-terminal Domain of the SARS CoV Nucleocapsid Protein"  J. Biomol. NMR 31, 79-80 (2005).

Assembly members:
nucleocapsid protein, polymer, 179 residues, Formula weight is not available

Natural source:   Common Name: SARS coronavirus   Taxonomy ID: 227859   Superkingdom: Viruses   Kingdom: not available   Genus/species: Coronavirus SARS coronavirus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):
nucleocapsid protein: MSDNGPQSNQRSAPRITFGG PTDSTDNNQNGGRNGARPKQ RRPQGLPNNTASWFTALTQH GKEELRFPRGQGVPINTNSG PDDQIGYYRRATRRVRGGDG KMKELSPRWYFYYLGTGPEA SLPYGANKEGIVWVATEGAL NTPKDHIGTRNPNNNAATVL QLPQGTTLPKGFYAEGSRG

Data sets:
Data typeCount
1H chemical shifts982
13C chemical shifts628
15N chemical shifts177

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SARS N-protein1

Entities:

Entity 1, SARS N-protein 179 residues - Formula weight is not available

1   METSERASPASNGLYPROGLNSERASNGLN
2   ARGSERALAPROARGILETHRPHEGLYGLY
3   PROTHRASPSERTHRASPASNASNGLNASN
4   GLYGLYARGASNGLYALAARGPROLYSGLN
5   ARGARGPROGLNGLYLEUPROASNASNTHR
6   ALASERTRPPHETHRALALEUTHRGLNHIS
7   GLYLYSGLUGLULEUARGPHEPROARGGLY
8   GLNGLYVALPROILEASNTHRASNSERGLY
9   PROASPASPGLNILEGLYTYRTYRARGARG
10   ALATHRARGARGVALARGGLYGLYASPGLY
11   LYSMETLYSGLULEUSERPROARGTRPTYR
12   PHETYRTYRLEUGLYTHRGLYPROGLUALA
13   SERLEUPROTYRGLYALAASNLYSGLUGLY
14   ILEVALTRPVALALATHRGLUGLYALALEU
15   ASNTHRPROLYSASPHISILEGLYTHRARG
16   ASNPROASNASNASNALAALATHRVALLEU
17   GLNLEUPROGLNGLYTHRTHRLEUPROLYS
18   GLYPHETYRALAGLUGLYSERARGGLY

Samples:

sample_1: nucleocapsid protein 1 mM

cond1: pH: 7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CCH-TOCSYnot availablenot availablenot available
HNCA-Dnot availablenot availablenot available
HN(CO)CA-Dnot availablenot availablenot available
HNCACB-Dnot availablenot availablenot available
HN(CO)CACB-Dnot availablenot availablenot available

Software:

NMRView v5.0.4 -

NMR spectrometers:

  • BRUKER AVANCE 800 MHz
  • BRUKER AVANCE 600 MHz
  • BRUKER AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAC81358 BAC81372 BAC81386 BAC81400 BAC81414
GB AAP13445 AAP13814 AAP30037 AAP30714 AAP33707
REF NP_828858
SP P59595 Q3I5I7

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts