BMRB Entry 6311

Title:
Sequence-specific backbone 1H, 13C and 15N assignments of the 25 kDa SPRY domain-containing SOCS box protein 2 (SSB-2)
Deposition date:
2004-09-01
Original release date:
2005-02-08
Authors:
Yao, Shenggen; Masters, Seth; Zhang, Jian-Guo; Palmer, Kristen; Babon, Jeffrey; Hilton, Douglas; Nicola, Nicos; Nicholson, Sandra; Norton, Raymond
Citation:

Citation: Yao, Shenggen; Masters, Seth; Zhang, Jian-Guo; Palmer, Kristen; Babon, Jeffrey; Nicola, Nicos; Nicholson, Sandra; Norton, Raymond. "Letter to the Editor: 1H, 13C and 15N assignments of the 25 kDa SPRY domain-containing SOCS box protein 2 (SSB-2)"  J. Biomol. NMR 31, 69-70 (2005).

Assembly members:

Assembly members:
Spry domain-containing SOCS box protein, polymer, 226 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Data sets:
Data typeCount
1H chemical shifts188
13C chemical shifts570
15N chemical shifts187

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SSB-21

Entities:

Entity 1, SSB-2 226 residues - Formula weight is not available

1   GLYSERSERALAARGGLNSERTHRPROTHR
2   SERGLNALALEUTYRSERASPPHESERPRO
3   PROGLUGLYLEUGLUGLULEULEUSERALA
4   PROPROPROASPLEUVALALAGLNARGHIS
5   HISGLYTRPASNPROLYSASPCYSSERGLU
6   ASNILEASPVALLYSGLUGLYGLYLEUCYS
7   PHEGLUARGARGPROVALALAGLNSERTHR
8   ASPGLYVALARGGLYLYSARGGLYTYRSER
9   ARGGLYLEUHISALATRPGLUILESERTRP
10   PROLEUGLUGLNARGGLYTHRHISALAVAL
11   VALGLYVALALATHRALALEUALAPROLEU
12   GLNALAASPHISTYRALAALALEULEUGLY
13   SERASNSERGLUSERTRPGLYTRPASPILE
14   GLYARGGLYLYSLEUTYRHISGLNSERLYS
15   GLYLEUGLUALAPROGLNTYRPROALAGLY
16   PROGLNGLYGLUGLNLEUVALVALPROGLU
17   ARGLEULEUVALVALLEUASPMETGLUGLU
18   GLYTHRLEUGLYTYRSERILEGLYGLYTHR
19   TYRLEUGLYPROALAPHEARGGLYLEULYS
20   GLYARGTHRLEUTYRPROSERVALSERALA
21   VALTRPGLYGLNCYSGLNVALARGILEARG
22   TYRMETGLYGLUARGARGVALGLUGLUTHR
23   ARGARGILEHISARGASP

Samples:

sample_1: Spry domain-containing SOCS box protein 0.60 mM

sample_2: Spry domain-containing SOCS box protein, [U-15N], 0.40 mM

sample_3: Spry domain-containing SOCS box protein, [U-13C; U-15N], 0.6 mM

sample_4: Spry domain-containing SOCS box protein, [U-13C; U-15N; U-70% 2H], 1.0 mM

Ex-cond_1: pH: 7.0; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available

Software:

XWINNMR v3.5 - spectral process

XEASY v1.3 - spectral analysis

NMR spectrometers:

  • Bruker AVANCE 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAE28397
GB AAC36017 AAH02005 AAH10305 AAL57356 EDK99761
REF NP_038567 XP_006505628 XP_006505629 XP_006505630 XP_006505631
SP O88838
AlphaFold O88838

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks