BMRB Entry 5937

Title:
1H, 13C and 15N Chemical Sift Assignments for PDZ2b domain of PTP-Bas (hPTP1E)
Deposition date:
2003-09-08
Original release date:
2003-12-19
Authors:
Kachel, Norman; Erdmann, Kai Sven; Kremer, Werner; Wolff, Peter; Gronwald, Wolfram; Heumann, Rolf; Kalbitzer, Hans Robert
Citation:

Citation: Kachel, Norman; Erdmann, Kai Sven; Kremer, Werner; Wolff, Peter; Gronwald, Wolfram; Heumann, Rolf; Kalbitzer, Hans Robert. "Structure determination and ligand interactions of the PDZ2b domain of PTP-Bas (hPTP1E): Splicing-induced modulation of ligand specificity"  J. Mol. Biol. 334, 143-155 (2003).
PubMed: 14596806

Assembly members:

Assembly members:
PDZ2b domain of PTP-Bas, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX2T

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts104
1H chemical shifts634

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ2b of PTP-Bas, PDZ2b1

Entities:

Entity 1, PDZ2b of PTP-Bas, PDZ2b 108 residues - Formula weight is not available

1   GLYSERSERPROPROLYSPROGLYASPILE
2   PHEGLUVALGLULEUALALYSASNASPASN
3   SERLEUGLYILESERVALTHRVALLEUPHE
4   ASPLYSGLYGLYVALASNTHRSERVALARG
5   HISGLYGLYILETYRVALLYSALAVALILE
6   PROGLNGLYALAALAGLUSERASPGLYARG
7   ILEHISLYSGLYASPARGVALLEUALAVAL
8   ASNGLYVALSERLEUGLUGLYALATHRHIS
9   LYSGLNALAVALGLUTHRLEUARGASNTHR
10   GLYGLNVALVALHISLEULEULEUGLULYS
11   GLYGLNSERPROTHRSERLYSGLU

Samples:

sample_1: PDZ2b domain of PTP-Bas, [U-13C; U-15N], 2 mM

sample_2: PDZ2b domain of PTP-Bas, [U-15N], 2 mM

sample_3: PDZ2b domain of PTP-Bas 2 mM

Con_1: pH: 6.8; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
15N-TOCSY-HSQCnot availablenot availablenot available
15N-NOESY-HSQCnot availablenot availablenot available
13C-NOESY-HSQCnot availablenot availablenot available
1H-1H-NOESYnot availablenot availablenot available

Software:

XWIN-NMR - data acquisition, processing

AUREMOL - automated assignment of NOESYs, manual assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks