BMRB Entry 5890

Title:
The NMR-derived Solution Structure of a New Cationic Antimicrobial Peptide from the Skin Secretion of the Anuran Hyla punctata
Deposition date:
2003-08-04
Original release date:
2004-02-12
Authors:
Prates, Maura; Regis, Wiliam; Sforca, Mauricio; Leite, Jose; Pertinhez, Thelma; Araujo, Antonio; Spisni, Alberto; Bloch, Carlos
Citation:

Citation: Prates, Maura; Regis, Wiliam; Sforca, Mauricio; Leite, Jose; Pertinhez, Thelma; Araujo, Antonio; Spisni, Alberto; Bloch, Carlos. "The NMR-derived Solution Structure of a New Cationic Antimicrobial Peptide from the Skin Secretion of the Anuran Hyla punctata"  J. Biol. Chem. 279, 13018-13026 (2004).
PubMed: 14715660

Assembly members:

Assembly members:
Hylaseptin P1, polymer, 14 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Polka-dot treefrog   Taxonomy ID: 280006   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hypsiboas punctatus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hylaseptin P1: GILDAIKAIAKAAG

Data sets:
Data typeCount
1H chemical shifts95
coupling constants12

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HSP1, Hylaseptin1

Entities:

Entity 1, HSP1, Hylaseptin 14 residues - Formula weight is not available

1   GLYILELEUASPALAILELYSALAILEALA
2   LYSALAALAGLY

Samples:

HSP1_sample: Hylaseptin P1 mM; 2,2,2-Trifluorethanol 30%; D2O 5%; H2O 95%

HSP1_cond: pH: 4; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H NOESYHSP1_samplenot availableHSP1_cond
2D 1H TOCSYHSP1_samplenot availableHSP1_cond
2D 1H COSYHSP1_samplenot availableHSP1_cond
2D 1H DQF-COSYHSP1_samplenot availableHSP1_cond

Software:

NMRpipe v5.0 - semi automated assignments

DYANA v1.5 - annealing

DISCOVER v2000 - energy minimization

ProcheckNMR v3.5.4 - analysis of the NMR restraints violations

CNSSOLVE v1.1 - analysis of the NMR restraints violations

NMRview v5.0 - semi automated assignments

NMR spectrometers:

  • Varian INOVA 500 MHz