BMRB Entry 5883

Title:
How C-Terminal Carboxyamidation Alters the Biological Activity of Peptides from the Venom of the Eumenine Solitary WasP
Deposition date:
2003-07-28
Original release date:
2008-07-16
Authors:
Sforca, Mauricio; Oyama, Sergio; Canduri, Fernanda; Lorenzi, Carla; Pertinhez, Thelma; Konno, Katsuhiro; Souza, Bibiana; Palma, Mario; Nero, Joao; Azevedo, Walter; Spisni, Alberto
Citation:

Citation: Sforca, Mauricio; Oyama Jr., Sergio; Canduri, Fernanda; Lorenzi, Carla; Pertinhez, Thelma; Konno, Katsuhiro; Souza, Bibiana; Palma, Mario; Nero, Joao; Azevedo Jr., Walter; Spisni, Alberto. "How C-Terminal Carboxyamidation Alters the Biological Activity of Peptides from the Venom of the Eumenine Solitary WasP"  Biochemistry 43, 5608-5617 (2004).
PubMed: 15134435

Assembly members:

Assembly members:
eumenine mastoparan Antherichium flavourmarginatum micado, polymer, 14 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Solitary wasp   Taxonomy ID: 329991   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Anterhynchium flavormarginatum micado flavomarginatum

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
eumenine mastoparan Antherichium flavourmarginatum micado: INLLKIAKGIIKSX

Data sets:
Data typeCount
1H chemical shifts85
coupling constants10

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1empaf, eumenine mastoparan1

Entities:

Entity 1, empaf, eumenine mastoparan 14 residues - Formula weight is not available

1   ILEASNLEULEULYSILEALALYSGLYILE
2   ILELYSSERNLW

Samples:

empaf_contents: eumenine mastoparan Antherichium flavourmarginatum micado 1.0 mM; 2,2,2-Trifluorethanol 30%

empaf_cond: pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H NOESYempaf_contentsnot availableempaf_cond
2D 1H TOCSYempaf_contentsnot availableempaf_cond
2D 1H COSYempaf_contentsnot availableempaf_cond
2D 1H DQF-COSYempaf_contentsnot availableempaf_cond

Software:

NMRpipe v5.0 - semi automated peak assignments

DISCOVER v2000 - energy minimization

DYANA v1.5 - annealing

ProcheckNMR v3.5.4 - analysis of NMR restraints violations

CNS v1.1 - analysis of NMR restraints violations

NMRview v5.0 - semi automated peak assignments

NMR spectrometers:

  • Varian INOVA 500 MHz