BMRB Entry 5836

Title:
Backbone 1H, 13C, 15N chemical shifts and coupling constants of Yersinia pseudotuberculosis-derived mitogen
Deposition date:
2003-06-17
Original release date:
2004-09-14
Authors:
Liew, Chu Wai; Donadini, Roberta; Fields, Barry; Mackay, Joel
Citation:

Citation: Liew, Chu Wai; Donadini, Roberta; Fields, Barry; Mackay, Joel. "Backbone 1H, 13C, 15N chemical shifts and coupling constants of Yersinia pseudotuberculosis-derived mitogen"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
Yersinia pseudotuberculosis-derived mitogen, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Yersinia pseudotuberculosis   Taxonomy ID: 633   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Yersinia pseudotuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMAL-p2x

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts154
15N chemical shifts91
1H chemical shifts709
coupling constants28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YPM1

Entities:

Entity 1, YPM 119 residues - Formula weight is not available

1   LEUARGILEPROASNILEALATHRTYRTHR
2   GLYTHRILEGLNGLYLYSGLYGLUVALCYS
3   ILEILEGLYASNLYSGLUGLYLYSTHRARG
4   GLYGLYGLULEUTYRALAVALLEUHISSER
5   THRASNVALASNALAASPMETTHRLEUILE
6   LEULEUARGASNVALGLYGLYASNGLYTRP
7   GLYGLUILELYSARGASNASPILEASPLYS
8   PROLEULYSTYRGLUASPTYRTYRTHRSER
9   GLYLEUSERTRPILETRPLYSILELYSASN
10   ASNSERSERGLUTHRSERASNTYRSERLEU
11   ASPALATHRVALHISASPASPLYSGLUASP
12   SERASPVALLEUTHRLYSCYSPROVAL

Samples:

sample_1: Yersinia pseudotuberculosis-derived mitogen, [U-95% 13C; U-90% 15N], 0.5 mM

sample_2: Yersinia pseudotuberculosis-derived mitogen 0.5 mM

Condition_1: ionic strength: 0 M; pH: 6.5 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available

Software:

XWINNMR v2.6 - raw spectral data processing

XEASY v1.3.13 - assignments, data analysis

DYANA v1.5 - structure refinement

ARIA v1.1.2 - structure refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA07528 BAA07619
GenBank AAK28542 AAL02230 ABS46381
REF YP_001400833