BMRB Entry 5754

Title:
1H, 13C and 15N backbone resonance assignment of the VASP EVH1 domain
Deposition date:
2003-03-24
Original release date:
2004-04-07
Authors:
Vogtherr, Martin; Grimme, Susanne; Pescatore, Barbara; Schwalbe, Harald
Citation:

Citation: Vogtherr, Martin; Grimme, Susanne; Pescatore, Barbara; Schwalbe, Harald. "Letter to the Editor: 1H, 13C and 15N Backbone Resonance Assignment of the VASP EVH1 Domain"  J. Biomol. NMR 27, 189-190 (2003).
PubMed: 12913419

Assembly members:

Assembly members:
Human VASP Ena-Vasp homology domain 1, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts110
1H chemical shifts460
13C chemical shifts332

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VASP EVH1 domain1

Entities:

Entity 1, VASP EVH1 domain 115 residues - Formula weight is not available

1   METSERGLUTHRVALILECYSSERSERARG
2   ALATHRVALMETLEUTYRASPASPGLYASN
3   LYSARGTRPLEUPROALAGLYTHRGLYPRO
4   GLNALAPHESERARGVALGLNILETYRHIS
5   ASNPROTHRALAASNSERPHEARGVALVAL
6   GLYARGLYSMETGLNPROASPGLNGLNVAL
7   VALILEASNCYSALAILEVALARGGLYVAL
8   LYSTYRASNGLNALATHRPROASNPHEHIS
9   GLNTRPARGASPALAARGGLNVALTRPGLY
10   LEUASNPHEGLYSERLYSGLUASPALAALA
11   GLNPHEALAALAGLYMETALASERALALEU
12   GLUALALEUGLUGLY

Samples:

sample_1: Human VASP Ena-Vasp homology domain 1, [U-13C; U-15N], 1.0 mM; DTT 10 mM; KCl 50 mM; KPi 20 mM

sample_2: Human VASP Ena-Vasp homology domain 1, [U-15N], 1.0 mM; DTT 10 mM; KCl 50 mM; KPi 20 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availableEx-cond_1
HN(CA)COnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
HCC(CO)NH-TOCSYnot availablenot availableEx-cond_1

Software:

xwinnmr v2.9 - acqusition, processing

xeasy v1.3.14 - peak assignment

talos v1999.019.15.47 - assignment, structure validation

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 18569
PDB
DBJ BAG37336 BAJ21098
EMBL CAA67147 CAA86523
GB AAH26019 AAH38224 AIC59345 EAW57362 EAW57363
REF NP_003361 XP_001106536 XP_002829453 XP_003277690 XP_003817563
SP P50552
AlphaFold P50552

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks