BMRB Entry 5711

Title:
Solution Structure of HP0371, Biotin Carboxyl Carrier protein from Helocobacter pylori
Deposition date:
2003-03-01
Original release date:
2009-03-17
Authors:
Jung, Jinwon; Lee, Weontae
Citation:

Citation: Jung, Jinwon; Lee, Chul-Jin; Jeon, Young Ho; Cheong, Chaejoon; Lee, Weontae. "Solution structure and backbone dynamics of the biotinylation domain of Helicobacter pylori biotin-carboxyl carrier protein"  B. Korean Chem. Soc. 29, 347-351 (2008).

Assembly members:

Assembly members:
Biotinoyl domain of HP0371, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Helicobater pylori   Taxonomy ID: not available   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Helicobater pylori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Biotinoyl domain of HP0371: GSVLSPMVGTFYHAPSPGAE PYVKAGDTLKKGQIVGIVEA MKIMNEIEVEYPCKVVSVEV GDAQPVEYGTKLIKVEKL

Data sets:
Data typeCount
13C chemical shifts211
15N chemical shifts68
1H chemical shifts440

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Biotinoyl domain of HP03711

Entities:

Entity 1, Biotinoyl domain of HP0371 78 residues - Formula weight is not available

1   GLYSERVALLEUSERPROMETVALGLYTHR
2   PHETYRHISALAPROSERPROGLYALAGLU
3   PROTYRVALLYSALAGLYASPTHRLEULYS
4   LYSGLYGLNILEVALGLYILEVALGLUALA
5   METLYSILEMETASNGLUILEGLUVALGLU
6   TYRPROCYSLYSVALVALSERVALGLUVAL
7   GLYASPALAGLNPROVALGLUTYRGLYTHR
8   LYSLEUILELYSVALGLULYSLEU

Samples:

sample_15N: Biotinoyl domain of HP0371, [U-95% 15N], 2 mM; sodium phosphate buffer 50 mM; NaCl 150 mM; DTT 1 mM

sample_13C_15N: Biotinoyl domain of HP0371, [U-95% 13C; U-95% 15N], 0.7 mM; sodium phosphate buffer 50 mM; NaCl 150 mM; DTT 1 mM

sample_13C: Biotinoyl domain of HP0371, [U-95% 13C], 0.4 mM; sodium phosphate buffer 50 mM; NaCl 150 mM; DTT 1 mM

Ex-cond_1: ionic strength: 0.3 M; pH: 7.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C CT-HSQCnot availablenot availablenot available
1H-15C TOCSYnot availablenot availablenot available
1H-15C NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

NMRPipe v2.1 - processing of data

SPARKY v3.106 - peak assignment, peak picking

AutoAssign v1.7 - automated backbond assignment

CYANA v1.0.6 - structure calculation with automated NOESY assignment

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian INOVA-UNITY-Plus 500 MHz

Related Database Links:

DBJ BAJ55623 BAJ56401 BAJ58603 BAJ60120 BAM96952
EMBL CAX29567 CBI65976
GB AAD07435 ABF85088 ACD48521 ACI27779 ACJ08202
REF NP_207169 WP_001053746 WP_001053747 WP_001053748 WP_001053749

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks