BMRB Entry 5593

Name: Backbone resonance assignments for the rat GMEB-2 SAND domain
Published: 2003-03-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5593

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignments for the rat GMEB-2 SAND domain

Deposition date:

2002-11-19

Original release date:

2003-03-18

Authors:

Bottomley, Matthew; Sattler, Michael

Citation:

Citation: Bottomley, Matthew; Sattler, Michael. "Letter to the Editor: 1H, 13C and 15N backbone resonance assignments of the SAND domains from glucocorticoid modulatory element binding proteins-1 and -2" J. Biomol. NMR 25, 259-260 (2003).

Assembly members:

Assembly members:
SAND domain from rat GMEB-2 protein, polymer, 101 residues, 11536 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Modified pET-24d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SAND domain from rat GMEB-2 protein: GAMEGENLEAEIVYPITCGD SRANLIWRKFVCPGINVKCV QYDEHVISPKEFVHLAGKST LKDWKRAIRMNGIMLRKIMD SGELDFYQHDKVCSNTCRST K

Data sets:

assigned_chemical_shifts
- CSR_1

Data type	Count
13C chemical shifts	195
15N chemical shifts	99
1H chemical shifts	99

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rat GMEB-2 extended SAND domain	1
2	ZINC (II) ION	2

Entities:

Entity 1, Rat GMEB-2 extended SAND domain 101 residues - 11536 Da.

1

GLY

ALA

MET

GLU

GLY

GLU

ASN

LEU

GLU

ALA

2

GLU

ILE

VAL

TYR

PRO

ILE

THR

CYS

GLY

ASP

3

SER

ARG

ALA

ASN

LEU

ILE

TRP

ARG

LYS

PHE

4

VAL

CYS

PRO

GLY

ILE

ASN

VAL

LYS

CYS

VAL

5

GLN

TYR

ASP

GLU

HIS

VAL

ILE

SER

PRO

LYS

6

GLU

PHE

VAL

HIS

LEU

ALA

GLY

LYS

SER

THR

7

LEU

LYS

ASP

TRP

LYS

ARG

ALA

ILE

ARG

MET

8

ASN

GLY

ILE

MET

LEU

ARG

LYS

ILE

MET

ASP

9

SER

GLY

GLU

LEU

ASP

PHE

TYR

GLN

HIS

ASP

10

LYS

VAL

CYS

SER

ASN

THR

CYS

ARG

SER

THR

11

LYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1

ZN

Samples:

Sample_1: SAND domain from rat GMEB-2 protein, [U-98% 15N], 1.0 mM; sodium phosphate 20 mM; NaCl 0.2 M; DTT 3 mM; H2O 90%; D2O 10%

Sample_2: SAND domain from rat GMEB-2 protein, [U-98% 13C; U-98% 15N], 1.0 mM; sodium phosphate 20 mM; NaCl 0.2 M; DTT 3 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 0.22 M; pH: 6.4; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	condition_1
3D HNCA	not available	not available	condition_1
3D CBCANH	not available	not available	condition_1
3D CBCA(CO)NH	not available	not available	condition_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks