BMRB Entry 5589

Title:
Backbone and side chain 1H, 13C, and 15N chemical shift assignments for V. cholerae VC0424
Deposition date:
2002-11-14
Original release date:
2003-06-23
Authors:
Ramelot, Theresa; Cort, John; Ni, Shuisong; Kennedy, Michael
Citation:

Citation: Ramelot, Theresa; Ni, S.; Goldsmith-Fischman, S.; Cort, J.; Honig, B.; Kennedy, M.. "Solution structure of Vibrio cholorae protein VC0424: A variation of the Ferredoxin-like fold"  Protein Sci. 12, 1562-1566 (2003).

Assembly members:

Assembly members:
VC0424, polymer, 132 residues, 15324 Da.

Natural source:

Natural source:   Common Name: V. cholerae   Taxonomy ID: 666   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Vibrio cholerae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28b

Data sets:
Data typeCount
1H chemical shifts863
13C chemical shifts552
15N chemical shifts130

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VC04241

Entities:

Entity 1, VC0424 132 residues - 15324 Da.

1   METSERHISGLNASPASPTYRLEUSERVAL
2   GLUGLULEUILEGLUILEGLNLYSGLUGLU
3   THRARGASPILEILEGLNALALEULEUGLU
4   ASPGLYSERASPPROASPALALEUTYRGLU
5   ILEGLUHISHISLEUPHEALAGLUASPPHE
6   ASPLYSLEUGLULYSALAALAVALGLUALA
7   PHELYSMETGLYPHEGLUVALLEUGLUALA
8   GLUGLUTHRGLUASPGLUASPGLYASNLYS
9   LEULEUCYSPHEASPALATHRMETGLNSER
10   ALALEUASPALALYSLEUILEASPGLUGLN
11   VALGLULYSLEUVALASNLEUALAGLULYS
12   PHEASPILEILETYRASPGLYTRPGLYTHR
13   TYRTYRGLUGLYLEUGLUHISHISHISHIS
14   HISHIS

Samples:

sample_1: VC0424, [U-15N; U-13C], 1 – 2 mM; Tris buffer 20 mM; NaCl 500 mM

cond_1: pH: 7.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availablecond_1
1H-13C HSQC (aliph, no CT)sample_1not availablecond_1
15N-edited NOESY-HQSC (80 ms)sample_1not availablecond_1
HCC-TOCSY-NNHsample_1not availablecond_1
HCCH-TOCSYsample_1not availablecond_1
13C-edited NOESY-HSQC (aliph)sample_1not availablecond_1
CBCACOCAHAsample_1not availablecond_1
HNCOsample_1not availablecond_1
HNCACBsample_1not availablecond_1
CBCA(CO)NHsample_1not availablecond_1
CC-TOCSY-NNHsample_1not availablecond_1
HNHAsample_1not availablecond_1
HNHBsample_1not availablecond_1
4D CC-NOESYsample_1not availablecond_1
1H-13C HSQC (aliph, CT)sample_1not availablecond_1
1H-13C HSQC (alpha, no CT)sample_1not availablecond_1
1H-13C HSQC (alpha, CT)sample_1not availablecond_1
1H-13C HSQC (arom, CT)sample_1not availablecond_1
13C-edited NOESY-HSQC (arom)sample_1not availablecond_1
15N-edited NOESY-HQSC (150 ms)sample_1not availablecond_1

Software:

Felix v98 - processing data

XPLOR v3.84 - structure calculations

Sparky v3.98 - chemical shift and noesy assignments

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ BAP01933
GB AAF93597 ABQ21042 ACP04735 ACP08488 ACQ59373
REF NP_230078 WP_000019330 WP_000019331 WP_000019332 WP_000019333

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks