BMRB Entry 5572

Title:
Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect
Deposition date:
2002-11-04
Original release date:
2002-12-27
Authors:
Graether, Steffen; Kuiper, Michael; Gagne, Stephane; Walker, Virginia; Jia, Zongchao; Sykes, Brian; Davies, Peter
Citation:

Citation: Graether, Steffen; Kuiper, Michael; Gagne, Stephane; Walker, Virginia; Jia, Zongchao; Sykes, Brian; Davies, Peter. "Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect"  Nature 406, 325-328 (2000).
PubMed: 10917537

Assembly members:

Assembly members:
spruce budworm antifreeze protein, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Spruce budworm   Taxonomy ID: 7141   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Choristoneura fumiferana

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts226
15N chemical shifts93
1H chemical shifts495

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sbwAFP1

Entities:

Entity 1, sbwAFP 90 residues - Formula weight is not available

1   ASPGLYSERCYSTHRASNTHRASNSERGLN
2   LEUSERALAASNSERLYSCYSGLULYSSER
3   THRLEUTHRASNCYSTYRVALASPLYSSER
4   GLUVALTYRGLYTHRTHRCYSTHRGLYSER
5   ARGPHEASPGLYVALTHRILETHRTHRSER
6   THRSERTHRGLYSERARGILESERGLYPRO
7   GLYCYSLYSILESERTHRCYSILEILETHR
8   GLYGLYVALPROALAPROSERALAALACYS
9   LYSILESERGLYCYSTHRPHESERALAASN

Samples:

sample_1: spruce budworm antifreeze protein, [U-15N; U-13C], 18 mM

condition_1: pH: 5.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 5573
PDB
GB AAG32660 AAG33032 AAG33033

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks