BMRB Entry 5563

Title:
Assignment of backbone 1H, 13C and 15N resonances of ParG, a protein required for active partition of bacterial plasmids
Deposition date:
2002-10-18
Original release date:
2003-12-08
Authors:
Golovanov, Alexander; Barilla, Daniela; Golovanova, Marina; Hayes, Finbarr; Lian, Lu-Yun
Citation:

Citation: Golovanov, Alexander; Barilla, Daniela; Golovanova, Marina; Hayes, Finbarr; Lian, Lu-Yun. "ParG, a protein required for active partition of bacterial plasmids, has a dimeric ribbon-helix-helix structure"  Mol. Microbiol. 50, 1141-1153 (2003).
PubMed: 14622405

Assembly members:

Assembly members:
ParG, polymer, 76 residues, 8625 Da.

Natural source:

Natural source:   Common Name: Salmonella enterica subsp. enterica serovar Newport   Taxonomy ID: 108619   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Salmonella enterica

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts83
1H chemical shifts513

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ParG subunit_11
2ParG subunit_21

Entities:

Entity 1, ParG subunit_1 76 residues - 8625 Da.

1   METSERLEUGLULYSALAHISTHRSERVAL
2   LYSLYSMETTHRPHEGLYGLUASNARGASP
3   LEUGLUARGVALVALTHRALAPROVALSER
4   SERGLYLYSILELYSARGVALASNVALASN
5   PHEASPGLUGLULYSHISTHRARGPHELYS
6   ALAALACYSALAARGLYSGLYTHRSERILE
7   THRASPVALVALASNGLNLEUVALASPASN
8   TRPLEULYSGLUASNGLU

Samples:

sample_1: ParG, [U-95% 15N], 1.0 – 2.0 mM

sample_2: ParG, [U-95% 13C; U-95% 15N], 1.0 – 2.0 mM

ex-cond_1: pH: 5.5 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available
2D 1H-1H COSY (in D2O)not availablenot availablenot available
2D 1H-1H TOCSY (in D2O)not availablenot availablenot available
2D 1H-1H NOESY (in D2O)not availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D CBCANHnot availablenot availablenot available
3D HAHB(CO)NHnot availablenot availablenot available
3D 15N NOESY-HSQCnot availablenot availablenot available
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablenot available
3D 15N TOCSY-HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GenBank AAF74218 ABZ89622 ACA51142
REF YP_001693222 YP_001716185 ZP_02351781

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks