BMRB Entry 5497

Title:
1H, 15N, 13C resonance assignments of the carboxy-terminal domain of the transmembrane electron transfer protein DsbD
Deposition date:
2002-08-08
Original release date:
2002-12-23
Authors:
Bushell, Mark; Ferguson, Stuart; Redfield, Christina
Citation:

Citation: Bushell, K.; Ferguson, Stuart; Redfield, Christina. "Letter to the Editor: 1H, 15N and 13C assignments of the carboxy-terminal domain of the transmembrane electron transfer protein DsbD"  J. Biomol. NMR 24, 359-360 (2002).

Assembly members:

Assembly members:
E. coli DsbD C-terminal domain, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b(+)

Data sets:
Data typeCount
1H chemical shifts719
13C chemical shifts469
15N chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DsbD C-terminal domain1

Entities:

Entity 1, DsbD C-terminal domain 160 residues - Formula weight is not available

1   METASPILEGLYILEASNSERASPPROVAL
2   ARGPROLEUGLNASPTRPALAPHEGLYALA
3   THRHISTHRALAGLNTHRGLNTHRHISLEU
4   ASNPHETHRGLNILELYSTHRVALASPGLU
5   LEUASNGLNALALEUVALGLUALALYSGLY
6   LYSPROVALMETLEUASPLEUTYRALAASP
7   TRPCYSVALALACYSLYSGLUPHEGLULYS
8   TYRTHRPHESERASPPROGLNVALGLNLYS
9   ALALEUALAASPTHRVALLEULEUGLNALA
10   ASNVALTHRALAASNASPALAGLNASPVAL
11   ALALEULEULYSHISLEUASNVALLEUGLY
12   LEUPROTHRILELEUPHEPHEASPGLYGLN
13   GLYGLNGLUHISPROGLNALAARGVALTHR
14   GLYPHEMETASPALAGLUTHRPHESERALA
15   HISLEUARGASPARGGLNPROLYSLEUALA
16   ALAALALEUGLUHISHISHISHISHISHIS

Samples:

sample1: E. coli DsbD C-terminal domain, [U-99% 15N], 1.5 mM

sample_2: E. coli DsbD C-terminal domain, [U-95% 13C; U-99% 15N], 1.5 mM

condition_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
The home-built spectrometers are equipped with Oxford Instrumentsnot availablenot availablecondition_1
Company magnets, Omega software and digital control equipmentnot availablenot availablecondition_1
(Bruker), home-built triple resonance pulsed field gradient probenot availablenot availablecondition_1
heads and home-built linear amplifiers.not availablenot availablecondition_1

Software:

FELIX v2.3 - data processing

NMR spectrometers:

  • home built . 600 MHz
  • home built . 750 MHz

Related Database Links:

PDB
DBJ BAB38540 BAE78138 BAG66862 BAG79959 BAI28440
EMBL CAA54781 CAA85375 CAP78654 CAQ34485 CAR01112
GB AAA97035 AAC77096 AAG59335 AAL50628 AAN45708
REF NP_290769 NP_313144 NP_418559 NP_710001 NP_757066
SP P36655 P58162 Q0SXE3 Q0T9Q5 Q1R3C4
AlphaFold P36655 P58162 Q0SXE3 Q0T9Q5 Q1R3C4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks