BMRB Entry 54037

Title:
mir377
Deposition date:
2026-09-28
Original release date:
2026-10-27
Authors:
Manigrasso, Jacopo; Mestdach, Emeline; Quaia, Riccardo; Dankova, Daniela; Bood, Mattias; Lamurell, Malin; Frolov, Andrey; D. Disney, Mathew; Hogner, Anders; Geschwindner, Stefan; Chi, Celestine; La Sala, Giuseppina
Citation:

Citation: Manigrasso, Jacopo; Mestdach, Emeline; Quaia, Riccardo; Dankova, Daniela; Bood, Mattias; Lamurell, Malin; Frolov, Andrey; D. Disney, Mathew; Hogner, Anders; Geschwindner, Stefan; Chi, Celestine; La Sala, Giuseppina. "Conformational dynamics at the pre-miR-377 Dicer site governs selective small-molecule recognition "  Nature ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 27 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGAAUUCGCUUUAUUUAUGU UGAAUCC

Data sets:
Data typeCount
13C chemical shifts62
15N chemical shifts11
1H chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mir3771

Entities:

Entity 1, mir377 27 residues - Formula weight is not available

1   GGAAUUCGCU
2   UUAUUUAUGU
3   UGAAUCC

Samples:

sample_1: mir377, [U-100% 15N], 1100 uM; mir377, [U-100% 13C], 1100 uM; DSS 10 uM

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz