Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR54027

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side chain chemical shift assignments of C-terminal domain of Bacillus subtilis NusG

Deposition date:

2026-09-18

Original release date:

2026-10-17

Authors:

Peterson, Reid; Tran, Thao; Sevugan, Swathi; Winkler, Wade; Fushman, David

Citation:

Citation: Tran, Thao; Peterson, Reid; Jiang, Xiaofang; Sevugan, Swathi; Ranaweera, Sandeepani; Babitzke, Paul; Fushman, David; Winkler, Wade. "The core interaction network of transcription elongation factors is broadly maintained across diverse bacteria " mBio ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 59 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: T7 express

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KTDIDFELKETVKVIDGPFA NFTGSIEEIDYDKSKVKVFV NMFGRETPVELEFTQIDKL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1
- spectral_peak_list_2

Data type	Count
15N chemical shifts	53
1H chemical shifts	281

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Bacillus subtilis NusG CTD	1

Entities:

Entity 1, Bacillus subtilis NusG CTD 59 residues - Formula weight is not available

1

LYS

THR

ASP

ILE

ASP

PHE

GLU

LEU

LYS

GLU

2

THR

VAL

LYS

VAL

ILE

ASP

GLY

PRO

PHE

ALA

3

ASN

PHE

THR

GLY

SER

ILE

GLU

ILE

ASP

4

TYR

ASP

LYS

SER

LYS

VAL

LYS

VAL

PHE

VAL

5

ASN

MET

PHE

GLY

ARG

GLU

THR

PRO

VAL

GLU

6

LEU

GLU

PHE

THR

GLN

ILE

ASP

LYS

LEU

Samples:

sample_1: Bacillus subtilis NusG CTD, [U-100% 15N], 400 uM; TRIS 25 mM; sodium chloride 100 mM; TCEP 1 mM; DSS 100 uM

sample_conditions_1: ionic strength: 0.1 M; pH: 8; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HMQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

TOPSPIN - collection

NMRFAM-SPARKY - peak picking

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 54027