BMRB Entry 54026
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR54026
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Tran, Thao; Peterson, Reid; Jiang, Xiaofang; Sevugan, Swathi; Ranaweera, Sandeepani; Babitzke, Paul; Fushman, David; Winkler, Wade. "The core interaction network of transcription elongation factors is broadly maintained across diverse bacteria
" mBio ., .-..
Assembly members:
entity_1, polymer, 131 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: T7 express
Entity Sequences (FASTA):
entity_1: MKRRTAREKALQALFQIDVS
DIAVNEAIEHALDEEKTDPF
FEQLVHGVLEHQDQLDEMIS
KHLVNWKLDRIANVDRAILR
LAAYEMAYAEDIPVNVSMNE
AIELAKRFGDDKATKFVNGV
LSNIKSDIEQS
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 256 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 419 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Bacillus subtilis NusB | 1 |
Entities:
Entity 1, Bacillus subtilis NusB 131 residues - Formula weight is not available
| 1 | MET | LYS | ARG | ARG | THR | ALA | ARG | GLU | LYS | ALA | ||||
| 2 | LEU | GLN | ALA | LEU | PHE | GLN | ILE | ASP | VAL | SER | ||||
| 3 | ASP | ILE | ALA | VAL | ASN | GLU | ALA | ILE | GLU | HIS | ||||
| 4 | ALA | LEU | ASP | GLU | GLU | LYS | THR | ASP | PRO | PHE | ||||
| 5 | PHE | GLU | GLN | LEU | VAL | HIS | GLY | VAL | LEU | GLU | ||||
| 6 | HIS | GLN | ASP | GLN | LEU | ASP | GLU | MET | ILE | SER | ||||
| 7 | LYS | HIS | LEU | VAL | ASN | TRP | LYS | LEU | ASP | ARG | ||||
| 8 | ILE | ALA | ASN | VAL | ASP | ARG | ALA | ILE | LEU | ARG | ||||
| 9 | LEU | ALA | ALA | TYR | GLU | MET | ALA | TYR | ALA | GLU | ||||
| 10 | ASP | ILE | PRO | VAL | ASN | VAL | SER | MET | ASN | GLU | ||||
| 11 | ALA | ILE | GLU | LEU | ALA | LYS | ARG | PHE | GLY | ASP | ||||
| 12 | ASP | LYS | ALA | THR | LYS | PHE | VAL | ASN | GLY | VAL | ||||
| 13 | LEU | SER | ASN | ILE | LYS | SER | ASP | ILE | GLU | GLN | ||||
| 14 | SER |
Samples:
sample_1: Bacillus subtilis NusB, [U-100% 15N], 250 uM; TRIS 25 mM; sodium chloride 100 mM; TCEP 1 mM; DSS 100 uM
sample_2: Bacillus subtilis NusB, [U-100% 13C; U-100% 15N], 250 uM; TRIS 25 mM; sodium chloride 100 mM; TCEP 1 mM; DSS 100 uM
sample_conditions_1: ionic strength: 0.1 M; pH: 8; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_2 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
TOPSPIN - collection
NMRFAM-SPARKY - peak picking
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks