BMRB Entry 54003

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR54003

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Title:
Assignment of Phosphorylated LIC2 C-terminal region
Deposition date:
2026-09-03
Original release date:
2026-09-11
Authors:
Warchol, Michael; Henen, Morkos; Vogeli, Beat
Citation:

Citation: Warchol, Michael; Henen, Morkos; Vogeli, Beat. "Phosphorylation in the Dynein LIC2 isoform enables Pin1 binding and modulates cargo-adaptor interactions "  JSB ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: WATPTRASESPARGPSGSPR TQGRGGPASVPSSSPGTSVK KPDPNIKNNAASEGVLASFF NSLLSKKTGSPGSPGAGGVQ STAKKSGQKTVLSNVQEELD RMTRKPDSMVTNSSTENEAG SGSGHHHHHH

Data sets:
Data typeCount
13C chemical shifts236
15N chemical shifts110
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pLIC2 C-terminus1

Entities:

Entity 1, pLIC2 C-terminus 130 residues - Formula weight is not available

1   TRPALATHRPROTHRARGALASERGLUSER
2   PROALAARGGLYPROSERGLYSERPROARG
3   THRGLNGLYARGGLYGLYPROALASERVAL
4   PROSERSERSERPROGLYTHRSERVALLYS
5   LYSPROASPPROASNILELYSASNASNALA
6   ALASERGLUGLYVALLEUALASERPHEPHE
7   ASNSERLEULEUSERLYSLYSTHRGLYSER
8   PROGLYSERPROGLYALAGLYGLYVALGLN
9   SERTHRALALYSLYSSERGLYGLNLYSTHR
10   VALLEUSERASNVALGLNGLUGLULEUASP
11   ARGMETTHRARGLYSPROASPSERMETVAL
12   THRASNSERSERTHRGLUASNGLUALAGLY
13   SERGLYSERGLYHISHISHISHISHISHIS

Samples:

sample_1: Phosphorylated LIC2 C terminus, [U-100% 13C; U-100% 15N], 200 uM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
HNNsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - chemical shift assignment, data analysis, peak picking

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks