BMRB Entry 53964

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53964

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Title:
1H, 13C, and 15N NMR backbone chemical shift assignments of the palladin Ig4 domain
Deposition date:
2026-08-07
Original release date:
2026-08-24
Authors:
Yadav, Rahul; Beck, Moriah
Citation:

Citation: Yadav, Rahul; Beck, Moriah. "1H, 13C, and 15N NMR backbone chemical shift assignments of the palladin Ig4 domain "  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 107 residues, 11840.51 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTBSG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SNAQERFFRPHFLQAPGDLT VQEGKLCRMDCKVSGLPTPD LSWQLDGKPIRPDSAHKMLV RENGVHSLIIEPVTSRDAGI YTCIATNRAGQNSFNLELVV AAKEAHK

Data sets:
Data typeCount
13C chemical shifts178
15N chemical shifts93
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1palladin Ig41

Entities:

Entity 1, palladin Ig4 107 residues - 11840.51 Da.

1   SERASNALAGLNGLUARGPHEPHEARGPRO
2   HISPHELEUGLNALAPROGLYASPLEUTHR
3   VALGLNGLUGLYLYSLEUCYSARGMETASP
4   CYSLYSVALSERGLYLEUPROTHRPROASP
5   LEUSERTRPGLNLEUASPGLYLYSPROILE
6   ARGPROASPSERALAHISLYSMETLEUVAL
7   ARGGLUASNGLYVALHISSERLEUILEILE
8   GLUPROVALTHRSERARGASPALAGLYILE
9   TYRTHRCYSILEALATHRASNARGALAGLY
10   GLNASNSERPHEASNLEUGLULEUVALVAL
11   ALAALALYSGLUALAHISLYS

Samples:

sample_1: palladin Ig4 domain, [U-100% 15N], 0.75 mM; HEPES 20 mM; D2O, [U-100% 2H], 10%; H2O 90%; sodium chloride 50 mM; TCEP 1 mM; sodium azide 0.05%

sample_2: palladin Ig4 domain, [U-99% 13C; U-99% 15N], 0.75 mM; HEPES 20 mM; D2O, [U-100% 2H], 10%; H2O 90%; sodium chloride 50 mM; TCEP 1 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.9; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(CO)CACBsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Related Database Links:

GB BAC39934.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks