BMRB Entry 5392

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5392
MolProbity Validation Chart

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Title:
Solution structure of HMG box 5 in human upstream binding factor
Deposition date:
2002-06-10
Original release date:
2003-12-08
Authors:
Yang, W.; Xu, Y.; Wu, J.; Zeng, W.; Shi, Y.
Citation:

Citation: Yang, W.; Xu, Y.; Wu, J.; Zeng, W.; Shi, Y.. "Solution structure and DNA binding property of the fifth HMG box domain in comparison with the first HMG box domain in human upstream binding factor"  Biochemistry 42, 1930-1938 (2003).
PubMed: 12590579

Assembly members:

Assembly members:
UBF1, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-22B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UBF1: GKLPESPKRAEEIWQQSVIG DYLARFKNDRVKALKAMEMT WNNMEKKEKLMWIKKAAEDQ KRYERELSEMRAPPAATNSS KKLE

Data sets:
Data typeCount
1H chemical shifts537
13C chemical shifts363
15N chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1upstream binding factor 11

Entities:

Entity 1, upstream binding factor 1 84 residues - Formula weight is not available

1   GLYLYSLEUPROGLUSERPROLYSARGALA
2   GLUGLUILETRPGLNGLNSERVALILEGLY
3   ASPTYRLEUALAARGPHELYSASNASPARG
4   VALLYSALALEULYSALAMETGLUMETTHR
5   TRPASNASNMETGLULYSLYSGLULYSLEU
6   METTRPILELYSLYSALAALAGLUASPGLN
7   LYSARGTYRGLUARGGLULEUSERGLUMET
8   ARGALAPROPROALAALATHRASNSERSER
9   LYSLYSLEUGLU

Samples:

sample1: UBF1, [U-15N; U-13C], 3 mM; NaPO4 45 mM; H20 90%; D20 10%

sample2: UBF1, [U-15N; U-13C], 3 mM; NaPO4 45 mM; D20 100%

sample_cond_1: pH: 5.5; temperature: 300 K; ionic strength: 45 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated-NOESYnot availablenot availablenot available
3D 13C-separated-NOESYnot availablenot availablenot available

Software:

NMRPipe - processing

PIPP - data analysis

CNS v1.0 - structure solution, refinement

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
DBJ BAB23701 BAE01971 BAE90957 BAF84422 BAF85207
EMBL CAA37469 CAA37548 CAA40016 CAA43222
GB AAB38418 AAB38419 AAC51239 AAH42297 EAW51616
PRF 1608205A
REF NP_001037848 NP_001070151 NP_001070152 NP_001099193 NP_001121162
SP P17480 P25976 P25977
AlphaFold P25977 P25977 P17480 P17480 P25976 P25976

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks