BMRB Entry 53873

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53873

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Title:
Mtb-NID in complex with PstC1-DNA template
Deposition date:
2026-06-16
Original release date:
2026-06-23
Authors:
Loth, Karine; Meudal, Herve
Citation:

Citation: DO, Thuy Duong; PINO PECO, Gabriel; EVENO, Eric; SAINT-ANDRE, Charlotte; DELALEAU, Mildred; COSTE, Franck; MEUDAL, Herve; LOTH, Karine; WEILXBAUMER, Albert; BRODOLIN, Konstantin; BOUDVILLAIN, Marc. "An intrinsically disordered domain of transcription terminator Rho governs genome-wide Rho utilization (Rut) site selection in GC-rich Mycobacterium tuberculosis "  Nucleic Acids Res. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 148 residues, Formula weight is not available
entity_2, polymer, 519 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMANGAPAVDRSAQEHDK GDRPPSSEAPATQGEQTPTE QIDSQSQQVRPERRSATREA GPSGSGERAGTAADDTDNRQ GGQQDAKTEERGTDAGGDQG GDQQASGGQQARGDEDGEAR QGRRGRRFRDRRRRGERSGD GAEAELRE
entity_2: CAGTCACGACGTTGTAAAAC GACGGCCAGTGAATTCACTG AAACTTGCCCGCTCGTTGAC TACTCGTGCGCAGTTGTGTT ACAATGGTCAGCGGCGGCCG CTCGTCCGCTCGCGCGCGAG TAGCCTCGTTGACCACCACG CGACAGCAACCTCCGTCGGG CCATCGGGCTGCTTTGCGGA GCATGCTGGCCCGTGCCGGT GAAGTCGGCCGCGCTGGCCC GGCCATCCGGTGGTTGGGTG GGATAGGTGCGGTGATCCCG CTGCTTGCGCTGGTCTTGGT GCTGGTGGTGCTGGTCATCG AGGCGATGGGTGCGATCAGG CTCAACGGGTTGCATTTCTT CACCGCCACCGAATGGAATC CAGGCAACACCTACGGCGAA ACCGTTGTCACCGACGGCGT CGCCCATCCGGTCGGCGCCT GCCCGACCGACAGCGCGCGA GTATTCACGCTGATATCAAC GCAAGCTTGGCGTAATCATG GTCATAGCTGTTTCCTGTGT GAAATTGTTATCCGCTCAC

Data sets:
Data typeCount

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NID1
2DNA2

Entities:

Entity 1, NID 148 residues - Formula weight is not available

1   GLYSERHISMETALAASNGLYALAPROALA
2   VALASPARGSERALAGLNGLUHISASPLYS
3   GLYASPARGPROPROSERSERGLUALAPRO
4   ALATHRGLNGLYGLUGLNTHRPROTHRGLU
5   GLNILEASPSERGLNSERGLNGLNVALARG
6   PROGLUARGARGSERALATHRARGGLUALA
7   GLYPROSERGLYSERGLYGLUARGALAGLY
8   THRALAALAASPASPTHRASPASNARGGLN
9   GLYGLYGLNGLNASPALALYSTHRGLUGLU
10   ARGGLYTHRASPALAGLYGLYASPGLNGLY
11   GLYASPGLNGLNALASERGLYGLYGLNGLN
12   ALAARGGLYASPGLUASPGLYGLUALAARG
13   GLNGLYARGARGGLYARGARGPHEARGASP
14   ARGARGARGARGGLYGLUARGSERGLYASP
15   GLYALAGLUALAGLULEUARGGLU

Entity 2, DNA 519 residues - Formula weight is not available

1   DCDADGDTDCDADCDGDADC
2   DGDTDTDGDTDADADADADC
3   DGDADCDGDGDCDCDADGDT
4   DGDADADTDTDCDADCDTDG
5   DADADADCDTDTDGDCDCDC
6   DGDCDTDCDGDTDTDGDADC
7   DTDADCDTDCDGDTDGDCDG
8   DCDADGDTDTDGDTDGDTDT
9   DADCDADADTDGDGDTDCDA
10   DGDCDGDGDCDGDGDCDCDG
11   DCDTDCDGDTDCDCDGDCDT
12   DCDGDCDGDCDGDCDGDADG
13   DTDADGDCDCDTDCDGDTDT
14   DGDADCDCDADCDCDADCDG
15   DCDGDADCDADGDCDADADC
16   DCDTDCDCDGDTDCDGDGDG
17   DCDCDADTDCDGDGDGDCDT
18   DGDCDTDTDTDGDCDGDGDA
19   DGDCDADTDGDCDTDGDGDC
20   DCDCDGDTDGDCDCDGDGDT
21   DGDADADGDTDCDGDGDCDC
22   DGDCDGDCDTDGDGDCDCDC
23   DGDGDCDCDADTDCDCDGDG
24   DTDGDGDTDTDGDGDGDTDG
25   DGDGDADTDADGDGDTDGDC
26   DGDGDTDGDADTDCDCDCDG
27   DCDTDGDCDTDTDGDCDGDC
28   DTDGDGDTDCDTDTDGDGDT
29   DGDCDTDGDGDTDGDGDTDG
30   DCDTDGDGDTDCDADTDCDG
31   DADGDGDCDGDADTDGDGDG
32   DTDGDCDGDADTDCDADGDG
33   DCDTDCDADADCDGDGDGDT
34   DTDGDCDADTDTDTDCDTDT
35   DCDADCDCDGDCDCDADCDC
36   DGDADADTDGDGDADADTDC
37   DCDADGDGDCDADADCDADC
38   DCDTDADCDGDGDCDGDADA
39   DADCDCDGDTDTDGDTDCDA
40   DCDCDGDADCDGDGDCDGDT
41   DCDGDCDCDCDADTDCDCDG
42   DGDTDCDGDGDCDGDCDCDT
43   DGDCDCDCDGDADCDCDGDA
44   DCDADGDCDGDCDGDCDGDA
45   DGDTDADTDTDCDADCDGDC
46   DTDGDADTDADTDCDADADC
47   DGDCDADADGDCDTDTDGDG
48   DCDGDTDADADTDCDADTDG
49   DGDTDCDADTDADGDCDTDG
50   DTDTDTDCDCDTDGDTDGDT
51   DGDADADADTDTDGDTDTDA
52   DTDCDCDGDCDTDCDADC

Samples:

sample_1: Mtb-NID, [U-99% 13C; U-99% 15N], 60 uM; PstC1-DNA template 25 uM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
So fast HMQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz