BMRB Entry 53834
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53834
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Xiang, Shengqi. "Condensation of MATCAP promotes CENP-E-dependent chromosome congression in mitosis
" Nat. Commun. ., .-..
Assembly members:
entity_1, polymer, 137 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: MVLDSGAQAYDQAPPSPPTS
PPSLRHRLKPSDRDGPPLYP
WSQSLALPLALAVPPALQPQ
PEQQPFSQMLLGHRGHMRRS
ESTYSVNSTGRRGRGTLGRP
PPGRGRNPGGGTLRPAASLP
HIAKTQRDAGHIASKSP
- spectral_peak_list
| Data type | Count |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IDR | 1 |
Entities:
Entity 1, IDR 137 residues - Formula weight is not available
| 1 | MET | VAL | LEU | ASP | SER | GLY | ALA | GLN | ALA | TYR | ||||
| 2 | ASP | GLN | ALA | PRO | PRO | SER | PRO | PRO | THR | SER | ||||
| 3 | PRO | PRO | SER | LEU | ARG | HIS | ARG | LEU | LYS | PRO | ||||
| 4 | SER | ASP | ARG | ASP | GLY | PRO | PRO | LEU | TYR | PRO | ||||
| 5 | TRP | SER | GLN | SER | LEU | ALA | LEU | PRO | LEU | ALA | ||||
| 6 | LEU | ALA | VAL | PRO | PRO | ALA | LEU | GLN | PRO | GLN | ||||
| 7 | PRO | GLU | GLN | GLN | PRO | PHE | SER | GLN | MET | LEU | ||||
| 8 | LEU | GLY | HIS | ARG | GLY | HIS | MET | ARG | ARG | SER | ||||
| 9 | GLU | SER | THR | TYR | SER | VAL | ASN | SER | THR | GLY | ||||
| 10 | ARG | ARG | GLY | ARG | GLY | THR | LEU | GLY | ARG | PRO | ||||
| 11 | PRO | PRO | GLY | ARG | GLY | ARG | ASN | PRO | GLY | GLY | ||||
| 12 | GLY | THR | LEU | ARG | PRO | ALA | ALA | SER | LEU | PRO | ||||
| 13 | HIS | ILE | ALA | LYS | THR | GLN | ARG | ASP | ALA | GLY | ||||
| 14 | HIS | ILE | ALA | SER | LYS | SER | PRO |
Samples:
sample_1: MATCAP IDR, [U-99% 15N], 0.1 mM; MATCAP IDR, [U-99% 15N], 0.7 mM; metallopeptidase domain 0.2 mM; PIPES 80 mM; MgCl2 1 mM; EGTA 1 mM; potassium chloride 100 mM; DTT 1 mM
sample_conditions_1: pH: 6.8; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - data analysis
NMR spectrometers:
- Bruker AVANCE III 600 MHz