Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53826

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H-15N Chemical shifts of Dsk2 dHS2 (deletion construct) from S. cerevisiae

Deposition date:

2026-05-28

Original release date:

2026-06-29

Authors:

Acharya, Nirbhik; Castaneda, Carlos

Citation:

Citation: Niblo, Jessica; Acharya, Nirbhik; Watkins, Maxwell; Castaneda, Carlos; Sukenik, Shahar. "Intramolecular interactions between folded and disordered regions shape ubiquilin structure and function " Adv. Sci. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 360 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pE-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSLNIHIKSGQDKWEVNVAP ESTVLQFKEAINKANGIPVA NQRLIYSGKILKDDQTVESY HIQDGHSVHLVKSQPKPQTA SAAGANNATATGAAAGTGAT PNMSSGQSAGFNPLADLTSA RYAGYLNMPSADMFGPDGGA LNNDSNNQDELLRMMENPIF QSQMNEMLSNPQMLDFMIQS NPQLQAMGPQARQMLQSPMF RQMLTNPDMIRQSMQFARMM DPNAGMGSAGGAASAFPAPG GDAPEEGSNTNTTSSSNTGN NAGTNAGTNAGANTAANPNA GNAASTGMPAFDPALLASMF QPPVQASQAEDTRPPEERYE HQLRQLNDMGFFDFDRNVAA LRRSGGSVQGALDSLLNGDV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	281
1H chemical shifts	281

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Dsk2 dHS2	1

Entities:

Entity 1, Dsk2 dHS2 360 residues - Formula weight is not available

The provided sequence is a Dsk2 variant (dHS2) lacking residues 279-291; author sequence numbering retains the wild-type scheme.

1	MET	SER	LEU	ASN	ILE	HIS	ILE	LYS	SER	GLY
2	GLN	ASP	LYS	TRP	GLU	VAL	ASN	VAL	ALA	PRO
3	GLU	SER	THR	VAL	LEU	GLN	PHE	LYS	GLU	ALA
4	ILE	ASN	LYS	ALA	ASN	GLY	ILE	PRO	VAL	ALA
5	ASN	GLN	ARG	LEU	ILE	TYR	SER	GLY	LYS	ILE
6	LEU	LYS	ASP	ASP	GLN	THR	VAL	GLU	SER	TYR
7	HIS	ILE	GLN	ASP	GLY	HIS	SER	VAL	HIS	LEU
8	VAL	LYS	SER	GLN	PRO	LYS	PRO	GLN	THR	ALA
9	SER	ALA	ALA	GLY	ALA	ASN	ASN	ALA	THR	ALA
10	THR	GLY	ALA	ALA	ALA	GLY	THR	GLY	ALA	THR
11	PRO	ASN	MET	SER	SER	GLY	GLN	SER	ALA	GLY
12	PHE	ASN	PRO	LEU	ALA	ASP	LEU	THR	SER	ALA
13	ARG	TYR	ALA	GLY	TYR	LEU	ASN	MET	PRO	SER
14	ALA	ASP	MET	PHE	GLY	PRO	ASP	GLY	GLY	ALA
15	LEU	ASN	ASN	ASP	SER	ASN	ASN	GLN	ASP	GLU
16	LEU	LEU	ARG	MET	MET	GLU	ASN	PRO	ILE	PHE
17	GLN	SER	GLN	MET	ASN	GLU	MET	LEU	SER	ASN
18	PRO	GLN	MET	LEU	ASP	PHE	MET	ILE	GLN	SER
19	ASN	PRO	GLN	LEU	GLN	ALA	MET	GLY	PRO	GLN
20	ALA	ARG	GLN	MET	LEU	GLN	SER	PRO	MET	PHE
21	ARG	GLN	MET	LEU	THR	ASN	PRO	ASP	MET	ILE
22	ARG	GLN	SER	MET	GLN	PHE	ALA	ARG	MET	MET
23	ASP	PRO	ASN	ALA	GLY	MET	GLY	SER	ALA	GLY
24	GLY	ALA	ALA	SER	ALA	PHE	PRO	ALA	PRO	GLY
25	GLY	ASP	ALA	PRO	GLU	GLU	GLY	SER	ASN	THR
26	ASN	THR	THR	SER	SER	SER	ASN	THR	GLY	ASN
27	ASN	ALA	GLY	THR	ASN	ALA	GLY	THR	ASN	ALA
28	GLY	ALA	ASN	THR	ALA	ALA	ASN	PRO	ASN	ALA
29	GLY	ASN	ALA	ALA	SER	THR	GLY	MET	PRO	ALA
30	PHE	ASP	PRO	ALA	LEU	LEU	ALA	SER	MET	PHE
31	GLN	PRO	PRO	VAL	GLN	ALA	SER	GLN	ALA	GLU
32	ASP	THR	ARG	PRO	PRO	GLU	GLU	ARG	TYR	GLU
33	HIS	GLN	LEU	ARG	GLN	LEU	ASN	ASP	MET	GLY
34	PHE	PHE	ASP	PHE	ASP	ARG	ASN	VAL	ALA	ALA
35	LEU	ARG	ARG	SER	GLY	GLY	SER	VAL	GLN	GLY
36	ALA	LEU	ASP	SER	LEU	LEU	ASN	GLY	ASP	VAL

Samples:

sample_1: Dsk2 dHS2, [U-99% 15N], 50 uM; sodium phosphate 20 mM; sodium azide 0.02%; EDTA 0.5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

CcpNMR v2.5.1 - data analysis

NMR spectrometers:

Bruker Avance 800 MHz

BMRB Entry 53826