BMRB Entry 53748

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53748

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Title:
Backbone 1H and 15N Chemical Shift Assignments for the CheA P1 domain from Escherichia coli with CheA-P3P4P5
Deposition date:
2026-04-30
Original release date:
2026-09-02
Authors:
Lu-Diaz, Katherine; Huang, Jian; Chen, Jianhan; Fejzo, Jasna; Thompson, Lynmarie
Citation:

Citation: Lu-Diaz, Katherine; Huang, Jian; Chen, Jianhan; Fejzo, Jasna; Thompson, Lynmarie. "Chemotaxis kinase CheA is regulated by modulating interdomain interactions "  Biophys. J. 125, 3261-3276 (2026).
PubMed: 42093153

Assembly members:

Assembly members:
entity_1, polymer, 134 residues, 15200 Da.
entity_2, polymer, 395 residues, 42800 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTEV-CheA-P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSMDISDFYQTFFDEADELL ADMEQHLLVLQPEAPDAEQL NAIFRAAHSIKGGAGTFGFS VLQETTHLMENLLDEARRGE MQLNTDIINLFLETKDIMQE QLDAYKQSQEPDAASFDYIC QALRQLALEAKGET
entity_2: ESTSIRVAVEKVDQLINLVG ELVITQSMLAQRSSELDPVN HGDLITSMGQLQRNARDLQE SVMSIRMMPMEYVFSRYPRL VRDLAGKLGKQVELTLVGSS TELDKSLIERIIDPLTHLVR NSLDHGIELPEKRLAAGKNS VGNLILSAEHQGGNICIEVT DDGAGLNRERILAKAASQGL TVSENMSDDEVAMLIFAPGF STAEQVTDVSGRGVGMDVVK RNIQKMGGHVEIQSKQGTGT TIRILLPLTLAILDGMSVRV ADEVFILPLNAVMESLQPRE ADLHPLAGGERVLEVRGEYL PIVELWKVFNVAGAKTEATQ GIVVILQSGGRRYALLVDQL IGQHQVVVKNLESNYRKVPG ISAATILGDGSVALIVDVSA LQAINREQRMANTAA

Data sets:
Data typeCount
15N chemical shifts106
1H chemical shifts106

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CheA P11
2CheA P3P4P52

Entities:

Entity 1, CheA P1 134 residues - 15200 Da.

1   METSERMETASPILESERASPPHETYRGLN
2   THRPHEPHEASPGLUALAASPGLULEULEU
3   ALAASPMETGLUGLNHISLEULEUVALLEU
4   GLNPROGLUALAPROASPALAGLUGLNLEU
5   ASNALAILEPHEARGALAALAHISSERILE
6   LYSGLYGLYALAGLYTHRPHEGLYPHESER
7   VALLEUGLNGLUTHRTHRHISLEUMETGLU
8   ASNLEULEUASPGLUALAARGARGGLYGLU
9   METGLNLEUASNTHRASPILEILEASNLEU
10   PHELEUGLUTHRLYSASPILEMETGLNGLU
11   GLNLEUASPALATYRLYSGLNSERGLNGLU
12   PROASPALAALASERPHEASPTYRILECYS
13   GLNALALEUARGGLNLEUALALEUGLUALA
14   LYSGLYGLUTHR

Entity 2, CheA P3P4P5 395 residues - 42800 Da.

1   GLUSERTHRSERILEARGVALALAVALGLU
2   LYSVALASPGLNLEUILEASNLEUVALGLY
3   GLULEUVALILETHRGLNSERMETLEUALA
4   GLNARGSERSERGLULEUASPPROVALASN
5   HISGLYASPLEUILETHRSERMETGLYGLN
6   LEUGLNARGASNALAARGASPLEUGLNGLU
7   SERVALMETSERILEARGMETMETPROMET
8   GLUTYRVALPHESERARGTYRPROARGLEU
9   VALARGASPLEUALAGLYLYSLEUGLYLYS
10   GLNVALGLULEUTHRLEUVALGLYSERSER
11   THRGLULEUASPLYSSERLEUILEGLUARG
12   ILEILEASPPROLEUTHRHISLEUVALARG
13   ASNSERLEUASPHISGLYILEGLULEUPRO
14   GLULYSARGLEUALAALAGLYLYSASNSER
15   VALGLYASNLEUILELEUSERALAGLUHIS
16   GLNGLYGLYASNILECYSILEGLUVALTHR
17   ASPASPGLYALAGLYLEUASNARGGLUARG
18   ILELEUALALYSALAALASERGLNGLYLEU
19   THRVALSERGLUASNMETSERASPASPGLU
20   VALALAMETLEUILEPHEALAPROGLYPHE
21   SERTHRALAGLUGLNVALTHRASPVALSER
22   GLYARGGLYVALGLYMETASPVALVALLYS
23   ARGASNILEGLNLYSMETGLYGLYHISVAL
24   GLUILEGLNSERLYSGLNGLYTHRGLYTHR
25   THRILEARGILELEULEUPROLEUTHRLEU
26   ALAILELEUASPGLYMETSERVALARGVAL
27   ALAASPGLUVALPHEILELEUPROLEUASN
28   ALAVALMETGLUSERLEUGLNPROARGGLU
29   ALAASPLEUHISPROLEUALAGLYGLYGLU
30   ARGVALLEUGLUVALARGGLYGLUTYRLEU
31   PROILEVALGLULEUTRPLYSVALPHEASN
32   VALALAGLYALALYSTHRGLUALATHRGLN
33   GLYILEVALVALILELEUGLNSERGLYGLY
34   ARGARGTYRALALEULEUVALASPGLNLEU
35   ILEGLYGLNHISGLNVALVALVALLYSASN
36   LEUGLUSERASNTYRARGLYSVALPROGLY
37   ILESERALAALATHRILELEUGLYASPGLY
38   SERVALALALEUILEVALASPVALSERALA
39   LEUGLNALAILEASNARGGLUGLNARGMET
40   ALAASNTHRALAALA

Samples:

sample_1: CheA P1, [U-2H; U-15N], 100 uM; D2O, [U-2H], 10%; H2O 90%; potassium chloride 75 mM; potassium phosphate 50 mM; magnesium chloride 5 mM; CheA-P3P4P5 500 uM

sample_conditions_1: ionic strength: 200 mM; pH: 7.5; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

CcpNMR - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks