BMRB Entry 53722

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53722

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Title:
Solution NMR assignments of the human CD7 ectodomain
Deposition date:
2026-04-15
Original release date:
2026-04-30
Authors:
Miles, Uri; Wu, Hongwei; Mcshan, Andrew
Citation:

Citation: Miles, Uri; Wu, Hongwei; Mcshan, Andrew. "A structural basis for T cell co-stimulation by the CD7 immunoreceptor and stimulatory ligand K12"  .

Assembly members:

Assembly members:
entity_1, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AQEVQQSPHCTTVPVGASVN ITCSTSGGLRGIYLRQLGPQ PQDIIYYEDGVVPTTDRRFR GRIDFSGSQDNLTITMHRLQ LSDTGTYTCQAITEVNVYGS GTLVLVTEEQSQGWHRCSDA

Data sets:
Data typeCount
13C chemical shifts319
15N chemical shifts104
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD7 ecto1

Entities:

Entity 1, CD7 ecto 120 residues - Formula weight is not available

1   ALAGLNGLUVALGLNGLNSERPROHISCYS
2   THRTHRVALPROVALGLYALASERVALASN
3   ILETHRCYSSERTHRSERGLYGLYLEUARG
4   GLYILETYRLEUARGGLNLEUGLYPROGLN
5   PROGLNASPILEILETYRTYRGLUASPGLY
6   VALVALPROTHRTHRASPARGARGPHEARG
7   GLYARGILEASPPHESERGLYSERGLNASP
8   ASNLEUTHRILETHRMETHISARGLEUGLN
9   LEUSERASPTHRGLYTHRTYRTHRCYSGLN
10   ALAILETHRGLUVALASNVALTYRGLYSER
11   GLYTHRLEUVALLEUVALTHRGLUGLUGLN
12   SERGLNGLYTRPHISARGCYSSERASPALA

Samples:

sample_1: CD7 ectodomain, [U-100% 13C; U-100% 15N], 0.45 mM; sodium azide 0.02%; sodium chloride 100 mM; sodium phosphate 20 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

TALOS-N - data analysis

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks