BMRB Entry 53712

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53712

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Title:
Backbone chemical shift assignment for monomeric 14-3-3zeta pS58
Deposition date:
2026-04-08
Original release date:
2026-07-21
Authors:
Kozelekova, Aneta; Crha, Radek; Brom, Tomas; Volko, Viliam; Hritz, Jozef
Citation:

Citation: Kozelekova, Aneta; Crha, Radek; Varecka, Vojtech; Brom, Tomas; Volko, Viliam; Kuty, Ondrej; Hritz, Jozef. "Structural and interaction analysis of monomeric 14-3-3z phosphorylated at Ser58 by NMR spectroscopy "  Int. J. Biol. Macromol. 375, 153383-153383 (2026).
PubMed: 42413672

Assembly members:

Assembly members:
entity_1, polymer, 248 residues, Formula weight is not available
entity_2, polymer, 248 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SVDMDKNELVQKAKEAEQAE RYDDMAAAMKSVTEQGAELS NEERNLLSVAYKNVVGARRS XWRVVSSIEQKTEGAEKKQQ MAREYREKIETELRDICNDV LSLLEKFLIPNASQAESKVF YLKMKGDYYRYLAEVAAGDD KKGIVDQSQQAYQEAFEISK KEMQPTHPIRLGLALNFSVF YYEILNSPEKAASLAKTAFD EAIAELDTLSEESYKDSTLI MQLLRDNLTLWTSDTQXDEA EAGXGGEN
entity_2: SVDMDKNELVQKAKEAEQAE RYDDMAAAMKSVTEQGAELS NEERNLLSVAYKNVVGARRS SWRVVSSIEQKTEGAEKKQQ KAREYREKIETELRDICNDV LSLLEKFLIPNASQAESKVF YLKMKGDYYRYLAEVAAGDD KKGIVDQSQQAYQEAFEISK KEMQPTHPIRLGLALNFSVF YYEILNSPEKAASLAKTAFD EAIAELDTLSEESYKDSTLI MQLLRDNLTLWTSDTQGDEA EAGEGGEN

Data sets:
Data typeCount
13C chemical shifts672
15N chemical shifts223
1H chemical shifts223

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomeric 14-3-3zeta L12E pS58 G234C E241C1
2monomeric 14-3-3zeta L12E M78K2

Entities:

Entity 1, monomeric 14-3-3zeta L12E pS58 G234C E241C 248 residues - Formula weight is not available

1   SERVALASPMETASPLYSASNGLULEUVAL
2   GLNLYSALALYSGLUALAGLUGLNALAGLU
3   ARGTYRASPASPMETALAALAALAMETLYS
4   SERVALTHRGLUGLNGLYALAGLULEUSER
5   ASNGLUGLUARGASNLEULEUSERVALALA
6   TYRLYSASNVALVALGLYALAARGARGSER
7   SEPTRPARGVALVALSERSERILEGLUGLN
8   LYSTHRGLUGLYALAGLULYSLYSGLNGLN
9   METALAARGGLUTYRARGGLULYSILEGLU
10   THRGLULEUARGASPILECYSASNASPVAL
11   LEUSERLEULEUGLULYSPHELEUILEPRO
12   ASNALASERGLNALAGLUSERLYSVALPHE
13   TYRLEULYSMETLYSGLYASPTYRTYRARG
14   TYRLEUALAGLUVALALAALAGLYASPASP
15   LYSLYSGLYILEVALASPGLNSERGLNGLN
16   ALATYRGLNGLUALAPHEGLUILESERLYS
17   LYSGLUMETGLNPROTHRHISPROILEARG
18   LEUGLYLEUALALEUASNPHESERVALPHE
19   TYRTYRGLUILELEUASNSERPROGLULYS
20   ALAALASERLEUALALYSTHRALAPHEASP
21   GLUALAILEALAGLULEUASPTHRLEUSER
22   GLUGLUSERTYRLYSASPSERTHRLEUILE
23   METGLNLEULEUARGASPASNLEUTHRLEU
24   TRPTHRSERASPTHRGLNXASPGLUALA
25   GLUALAGLYXGLYGLYGLUASN

Entity 2, monomeric 14-3-3zeta L12E M78K 248 residues - Formula weight is not available

1   SERVALASPMETASPLYSASNGLULEUVAL
2   GLNLYSALALYSGLUALAGLUGLNALAGLU
3   ARGTYRASPASPMETALAALAALAMETLYS
4   SERVALTHRGLUGLNGLYALAGLULEUSER
5   ASNGLUGLUARGASNLEULEUSERVALALA
6   TYRLYSASNVALVALGLYALAARGARGSER
7   SERTRPARGVALVALSERSERILEGLUGLN
8   LYSTHRGLUGLYALAGLULYSLYSGLNGLN
9   LYSALAARGGLUTYRARGGLULYSILEGLU
10   THRGLULEUARGASPILECYSASNASPVAL
11   LEUSERLEULEUGLULYSPHELEUILEPRO
12   ASNALASERGLNALAGLUSERLYSVALPHE
13   TYRLEULYSMETLYSGLYASPTYRTYRARG
14   TYRLEUALAGLUVALALAALAGLYASPASP
15   LYSLYSGLYILEVALASPGLNSERGLNGLN
16   ALATYRGLNGLUALAPHEGLUILESERLYS
17   LYSGLUMETGLNPROTHRHISPROILEARG
18   LEUGLYLEUALALEUASNPHESERVALPHE
19   TYRTYRGLUILELEUASNSERPROGLULYS
20   ALAALASERLEUALALYSTHRALAPHEASP
21   GLUALAILEALAGLULEUASPTHRLEUSER
22   GLUGLUSERTYRLYSASPSERTHRLEUILE
23   METGLNLEULEUARGASPASNLEUTHRLEU
24   TRPTHRSERASPTHRGLNGLYASPGLUALA
25   GLUALAGLYGLUGLYGLYGLUASN

Samples:

sample_1: 14-3-3zeta L12E pS58 G234C E241C + two 5-MSL spin labels, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 150 mM; sodium azide 3 mM; TCEP 2 mM; D2O, [U-100% 2H], 10%; H2O 90%

sample_2: 14-3-3zeta L12E M78K, [U-100% 13C; U-100% 15N], 0.4 mM; sodium phosphate 150 mM; sodium azide 3 mM; D2O, [U-100% 2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_2isotropicsample_conditions_2
3D HNCAsample_2isotropicsample_conditions_2
3D HN(CO)CAsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2
3D CBCA(CO)NHsample_2isotropicsample_conditions_2

Software:

TOPSPIN - collection

NMRFAM-SPARKY v1.470 - chemical shift assignment, data analysis, peak picking

NMRPipe v12.1 - processing

NMR spectrometers:

  • Bruker AVANCE NEO 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks