BMRB Entry 53708
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53708
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Lopez-Sanchez, Ruben; Pantoja-Uceda, David; Mompean, Miguel; Laurents, Douglas. "PolyProline Predictor: A Web Server for Empirical Sequence-Based Prediction of Polyproline II Helices" Prot. Sci. ., .-..
Assembly members:
entity_1, polymer, 11 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: XPGPRGNQGAX
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 17 |
| 1H chemical shifts | 40 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RIPK3 peptide PG | 1 |
Entities:
Entity 1, RIPK3 peptide PG 11 residues - Formula weight is not available
The peptide contains a N-terminal acetyl group and a C-terminal amide group.
| 1 | ACE | PRO | GLY | PRO | ARG | GLY | ASN | GLN | GLY | ALA | ||||
| 2 | NH2 |
Samples:
sample_1: RipK3 peptide PG 5.50 ± 0.2 mM; DSS 100 ± 5 uM; potassium phosphate 10 ± 1 mM; D2O 55 ± 1 M
sample_2: RipK3 peptide PG 2.20 ± 0.2 mM; DSS 100 ± 5 uM; potassium phosphate 10 ± 1 mM; D2O 22 ± 1 M; H2O 22 ± 1 M
sample_conditions_1: ionic strength: 50 mM; pH: 6.65; pressure: 0.93 atm; temperature: 278.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.1 - collection
NMRFAM-SPARKY v1.470 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz